Relationship research between fecal microbes and short chain fatty acid between wild yak and domestic yak
LIU Chuanfa, ZHANG Liangzhi, FU Haibo, LI Wenjing, ZHANG He, LI Jiye, PI Li，ZHANG Yanming
(1 Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China)
(2 University of Chinese Academy of Sciences, Beijing 100049, China)
(3 Datong Yak Breeding Farm of Qinghai Province, Datong 810102, China)
Short chain fatty acids (SCFA), are a crucial form of nutrition for digestion and assimilation of grass for ruminants including the yaks. Gut microbiota degrades chyme, produces SCFA and influences its proportion. Here, we investigated the fecal microbiota and SCFA concentration in wild yak (Bos mutus) and domestic yak (Bos grunniens). We hypothesize that yaks’ gut microbiota influence concentration of SCFA and in that way increase the assimilation of nutrients from the grass. Microbes and SCFA concentration of yaks were processed and measured by 16S rDNA and Gas Chromatography-Mass Spectrometry respectively. The results show that the yak fecal microbes are mainly composed of Firmicutes(66.47%, 68.38%)(wild yak and domestic yak respectively, the same below), Bacteroidetes(26.00%, 26.16%) and Proteobacteria(3.48%, 1.52%) at the phylum level. Furthermore, Ruminococcaceae(55.18%, 58.48%), Bacteroidaceae(8.75%, 9.59%) and Lachnospiraceae(7.57%, 6.14%) at the family level; the domestic yak has the similar mainly composition with wild yak. SCFA concentration (including acetic acid, propionic acid, i-butyric acid, n-butyric acid and n-valeric acid) in wild yaks’ feces are significantly higher than in domestic yak (P<0.01). Spearman correlation relationship analysis shows that there is strong positive correlation between Porphyromonadaceae, Bacteroidaceae, Paraprevotellaceae, Rikenellaceae, Erysipelotrichaceae and SCFA concentration in wild yaks (r>0.4, P<0.05). However, there only has weak positive correlation in domestic yak’s microbes. Our result suggests that the digestion of grass in wild yaks is more efficient than in domestic yaks.
刘传发 张良志 付海波 李文靖 张贺 李吉叶 皮立 张堰铭. 野牦牛和家牦牛粪便菌群与短链脂肪酸关系的研究[J]. 兽类学报, 2019, 39(1): 1-7.
LIU Chuanfa, ZHANG Liangzhi, FU Haibo, LI Wenjing, ZHANG He, LI Jiye, PI Li，ZHANG Yanming. Relationship research between fecal microbes and short chain fatty acid between wild yak and domestic yak. ACTA Theriologica Sinica, 2019, 39(1): 1-7.