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冷驯化及复温对中缅树鼩白色脂肪组织中Ucp1和Cd137表达的影响

陶洁琼1 贾婷2 刘璐3 朱万龙1 王政昆1 张浩1#br#   

  1. (1 云南省高校西南山地生态系统动植物生态适应进化及保护重点实验室,云南师范大学生命科学学院,生物能源持续开发利用教育部工程研究中心,云南省生物质能与环境生物技术重点实验室,昆明 650500)(2 云南经济管理学院,昆明 650106)(3昆明医科大学第二附属医院,昆明 650101)
     
  • 出版日期:2020-04-01 发布日期:2020-04-09
  • 通讯作者: 王政昆 E-mail: wangzk_123@163.com; 张浩 E-mail: aple20@sina.com

Effects of cold acclimation and rewarming on the expression of Ucp1 and Cd137 in white adipocytes tissue of Tupaia belangeri

TAO Jieqiong1, JIA Ting2, LIU Lu 3, ZHU Wanlong 1, WANG Zhengkun 1, ZHANG Hao 1#br#   

  1.  (1 Key Laboratory of Ecological Adaptive Evolution and Conservation on Animals-Plants in Southwest Mountain Ecosystem of Yunnan Province Higher Institutes College Kunming, School of Life Sciences of Yunnan Normal University, Engineering Research Center of Ministry of Education for Sustainable Development and Utilization of Bioenergy, Key Laboratory of Biomass Energy and Environmental Biotechnology in Yunnan Province, Kunming 650500, China)  (2 Yunnan College of Business Management, Kunming 650106,China)  (3 The second affiliated Hospital of Kunming Medical University, Kunming 650101,China)
  • Online:2020-04-01 Published:2020-04-09

摘要:

为探讨中缅树鼩(Tupaia belangeri) 白色脂肪组织(white adipose tissue, WAT)应对环境温度变化的适应机制,以荧光标记的Ucp1和Cd137抗体分别对中缅树鼩WAT进行标记,利用流式细胞术分选分析出对照组、冷驯化28d组、复温28d组阳性的白色脂肪细胞。结果发现:WAT中表达Ucp1、Cd137阳性的白色脂肪细胞百分数,在冷驯化组均显著高于对照组,而复温组均显著低于冷驯化组,恢复至对照组水平。表明:冷驯化诱导中缅树鼩WAT中表达Ucp1、Cd137的阳性细胞群增加,显示冷驯化能诱导中缅树鼩的WAT发生褐变;复温后表达Ucp1、Cd137阳性的白色脂肪细胞百分数恢复到对照水平,反映出WAT的可塑性。因此,WAT的可塑性调节也是中缅树鼩适应环境温度变化的重要产热机制。

关键词: 中缅树鼩, 白色脂肪组织, Cd137, Ucp1

Abstract:

In order to explore the adaptive mechanism that how does the white adipose tissue (WAT) of the tree shrews (Tupaia belangeri) reply to the change of the environmental temperature, the WAT of tree shrews, which came from three groups, the control group, cold acclimation group (28 day) and the rewarming group (28 day), were labeled by fluorescent labeled Ucp1 antibody and fluorescent labeled Cd137 antibody, and then were analyzed by the Flow cytometry to measure the white adipocytes which had Ucp1 and Cd137. As a result, the percentage of the white adipocytes which had Ucp1 and Cd137 in the cold acclimation group was higher than that in the control group significantly. Nevertheless, the percentage of the white adipocytes which had Ucp1 and Cd137 in the rewarming group was lower than that in the cold acclimation group significantly and returned to the percentage of that in the control group. The increase of the white adipocytes which had Ucp1 and Cd137 was observed in the tree shrews during cold acclimation. The browning of WAT in tree shrews could be induced during cold acclimation. The plasticity of WAT was reflected by that the percentage of the white adipocytes which had Ucp1 and Cd137 returned to what they once were after rewarming. Therefore, the plasticity regulation of WAT is also an important thermogenic mechanism for the tree shrews to adapt the changes of
ambient temperature.

Key words: Tupaia belangeri, white adipose tissue, Cd137, Ucp1