Abstract: To study the efficiency of methods for extracting urinary and fecal steroid hormones in Sichuan snub-nosed monkey ( Rhinopithecus roxellana), three extraction solvents and five extraction methodswere used to extract the hormones in urine and feces, respectively. The recovery rate of extractions was employed for comparison. The results showed that the recovery rates of the urinary testosterone in male monkey, the urinary estradiol and progesterone in female monkey, were 79.27%, 73.39% and 80.28%, respectively, with dichloromethane as extraction solvent. The results were higher than those applying the other two extraction solvents.When fecal testosterone and estradiol in male monkey, fecal testosterone, estradiol and progesterone in female monkeywere extracted by ethanol-heating method, the recovery rateswere71.33%, 85.12%, 67.41%, 79.58% and 77.71%, respectively.Weighed the five methods and ethanol-heating method was the best one. Furthermore, ethanol-heating method was simple and easy to be manipulated. The conclusion could be made that ichloromethane and ethanol-heating method were the efficient extraction solvent and way , respectively , for urinary and fecal steroid hormones in Sichuan snub-nosed monkey.

Key words: Dichloromethane, Extraction, Ethanol-heating method, Sichuan snub-nosed monkey, Steroid hormone, Recovery rate

摘要： 本文旨在探讨川金丝猴粪尿中类固醇性激素的抽提方法, 实验比较了3 种尿液抽提溶剂和5 种粪便抽提方法的抽提回收率。结果显示, 以二氯甲烷为溶剂抽提雄猴尿液中的睾酮、雌猴尿液中的雌二醇和孕酮, 回收率分别为79.27%、73.39%和80.28%, 抽提效果较其它两种溶剂为好; 以乙醇加热法抽提雄猴粪便中的睾酮和雌二醇、雌猴粪便中的睾酮、雌二醇和孕酮, 回收率分别为1.33%、85.12%与67.41%、79.58%和77.71%, 抽提效果优于其它4 种方法,且该方法简便易于操作。实验表明, 以二氯甲烷和乙醇加热法分别抽提川金丝猴尿液和粪便中的类固醇性激素是较理想的抽提方法。

关键词: 川金丝猴, 类固醇性激素, 抽提, 回收率, 二氯甲烷, 乙醇加热法