Previous Articles     Next Articles

Histological observations on ovaries of adult female red pandas (Ailurus fulgens)

XIU Yunfang   

  1. Fuzhou Giant Panda Research Center,Fuzhou 350001,China
  • Online:2010-08-31 Published:2010-03-02

成体雌性小熊猫卵巢组织学观察

修云芳   

  1. (福州大熊猫研究中心,福州 350001

Abstract: Twenty-four ovaries were collected from twelve adult female red pandas died of non-reproductive system diseases
at different stages of estrous cycles from 2000 to 2009. The ovaries were fixed in 10% formalin,and tissue slices were made using standard histological techniques and stained with HE. All slices were observed under optical microscopes. Results showed that (1) Primordial follicles,primary follicles and secondary follicles exist simultaneously in all ovaries of different stages of estrous cycles. Typical mature follicles and oocytes are not observed in the ovaries during the estrous stage. (2) There are more primary follicles than primordial follicles and most of primary follicles and secondary follicles are artresic in all ovaries. (3) Before follicular antrum formed, oocytes and follicles are developed synchronously,while in antral follicles,the development of follicles is faster than that of oocytes.(4) Large amounts of interstitial gland cells are observed obviously in ovaries at different estrous stages. (5) Well-developed corpus luteum is present in both the ovaries of pregnant and non-pregnant animals during diestrus period. (6) Zona peliucida is developed when oocytes are surrounded by single layer of cubical granulosa cells. Conclusions:(1)Follicular atresia occurs mainly in the primary follicle stage and only a few primary follicles can be developed to be secondary follicles. (2) Development of oocytes and follicles exhibits biphasic
growth trend. (3) Ovary interstitial gland cells are well developed in all stages of the estrous cycle. (4) Durations
of corpus luteum in pregnant animals are similar to those in non-pregnant animals,confirming the existence of pseudo-pregnancy of the animals.

Key words: Histology, Ovary, Red panda (Ailurus fulgens), Reproduction

摘要: 2000 年至2009 年,12 只固定于10% 福尔马林中非生殖系统疾病死亡的小熊猫卵巢组织,按常规组织学技术制作组织切片,HE 染色,光学显微镜观察。结果:(1)不同发情时期卵巢均有原始卵泡、初级卵泡和次级卵泡分布。发情期的卵巢未观察到典型的成熟卵泡和卵母细胞; (2)原始卵泡数量较少,初级卵泡数量较多,多数初级卵泡和大多数的次级卵泡都处在闭锁状态;(3)卵泡腔出现之前,卵母细胞的直径和卵泡直径同时增长;卵泡腔出现之后,卵母细胞直径增长较慢,卵泡直径增长较快; (4)不同发情时期的小熊猫卵巢均存在大量的间质腺细胞;(5)妊娠小熊猫和发情间期无妊娠小熊猫的卵巢均有发育正常的黄体;(6)卵泡细胞发育呈低柱状至柱状时出现透明带。结论:(1) 卵泡闭锁主要发生在初级卵泡阶段,仅少数卵泡能发育至次级卵泡;(2)卵母细胞和卵泡生长呈双相生长的趋势; (3) 不同发情时期的小熊猫卵巢间质腺都发达; (4)发情排卵后,非妊娠黄体与妊娠黄体维持的时间相似,证实了小熊猫存在假孕现象。

关键词: 小熊猫, 组织学, 卵巢, 繁殖