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A Preliminary Study of Plateau Pika ( Ochotona curzoniae) Hypoxia-Induced Factor-1α

ZHAO Tongbiao ZHAO Xinquan CHANG Zhijie ZHU Shanshan ZHAO Wei SUN Ping XU Shixiao   

  • Online:2006-08-16 Published:2008-07-07

高原鼠兔低氧诱导因子- 1α的初步研究

赵同标  赵新全  常智杰  朱姗姗  赵伟  孙平  徐世晓   

  1. 中国科学院西北高原生物研究所

Abstract: Hypoxia-induced factor-1 plays a key role during the cell hypoxia transduction. Hypoxia induced factor-1α (HIF-1α) is a functional subunit of hypoxia-induced factor-1. Plateau pika ( Ochotona curzoniae) , which is a Qinghai-Tibet plateau native animal lived above 3 000 m , has high ratio of oxygen utilization to adapt to plateau hypoxia environments. One fragment of the coding region of cDNA sequence of plateau pika HIF-1α was obtained by RT-PCR technique using a degeneracy PCR primer based on previously reported cDNA sequence in human , cattle , house mouse and Norway rat HIF-1αgene. It was directly inserted into the vector pMD18-T. DNA sequencing proved that it was highly homology with human (91%) , cattle (91%) , house mouse (89%) and Norway rat (89%) HIF-1α gene. The study provides basic important information for the HIF-1α cDNA whole sequence cloning of plateau pika and its functional study.

Key words: Plateau pika ( Ochotona curzoniae), HIF21, RT2PCR, Qinghai2Tibet Plateau

关键词: 低氧诱导因子-1α, 高原鼠兔, RT- PCR, 青藏高原