Abstract: It is inevitable to develop noninvasive sampling methods to do studies on giant panda even diagnose the diseases since which is so endangered that it's impossible to carry out invasive sampling. A non-invasive sampling method to detect the intestinal pathogen , Yersinia enterocolitica in feces of pandas based designing PCR primers was established in this study. The main procedures are based on bacteria enrichment and cell lysis before binding the pathogen DNA to silica powder at high concentration of Kalium iodide and neutral pH conditions. Before PCR cycles , the binded DNA is washed with 80% ethanol and eluted with diluted EDTA buffer. Results showed that the silica-based feces DNA-purification method could remove the inhibitors of PCR so applicable to detect the target pathogen.

Key words: Giant panda, Yersinia enterocolitica, Feces, Non-invasive sampling

关键词: 大熊猫, 小肠结肠炎耶尔森氏菌, 粪便, 非损伤性取样