Abstract: Chromosome preparations were made from culture cells of primary lung and tail tissue,then determination of the diploid number (2n) was performed on 30 metaphase cells from each specimen.The karyotypes of five species of Phinolophus bats from Guangdong,China were examined,namely: Phinolophus pusillus (2n=62，FN =60)，R. cornutus (2n=62,FN =60), R. affinis (2n=62，FN =60), R. macrotis(2n=62，FN =60), R. sinicus(2n=36，FN =60).The karyotypes of R.macrotis is reported for the first time. It is essentially identical to those of previous reports about the karyotypes of R.cornutus,and R.affinis. The karyotype of R.sinicus (2n=36) is same as R.rouxii sinicus from Anhui reported by Zhang Weidao, but different from that of R.rouxii (2n=56) from India and Sri Lanka. Finally Rhinolophus diversity in the East Asia is discussed．

Key words: Karyotype, Rhinolophus, Bats, Guangdong

摘要： 本文用蝙蝠的新鲜肺组织和尾椎进行组织培养，然后在光学显微镜下计数30个染色体分散良好的中期分裂相细胞，并进行摄影、剪贴和测量。分析了广东地区5种菊头蝠的核型，即小菊头蝠的核型为2n=62，FN=60；角菊头蝠的核型为2n：62，FN=60；中菊头蝠的核型为2n=62，FN：60；大耳菊头蝠的核型为2n=62，FN=60；中华(栗黄)菊头蝠的核型为2n=36，FN=60。大耳菊头蝠的核型为首次报道。角菊头蝠和中菊头蝠的核型与前人报道的结果基本相同。中华(栗黄)菊头蝠的核型(2n=36)与张维道报道的鲁氏菊头蝠相同，
而与印度和斯里兰卡产R.rouxii的核型2n=56迥异。最后对东亚地区菊头蝠多样性进行了探讨。

关键词: 核型, 菊头蝠, 蝙蝠, 广东