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THE PARENTAGE TEST 0F WILD H0USE MICE BY DNA FINGERPRINTING

FANG Jiming,Jane Hurst,Chris Barnard   

  • Online:2006-07-29 Published:2008-01-05

用DNA指纹图谱方法进行野生小家鼠的双亲判别

房继明,Jane Hurst, Chris Barnard, 孙儒泳   

Abstract: This is the first paper about the study of parentage test of wild house mice(Mus musculus domesticus)by DNA fingerprinting.Using DNA fingerprinting is able to identify the relationship between wild house mice offspring and their panents. In stead of the similarity coeficients,the comparison of band sharing was recommended to be applied for the parentage test of wild house mice.because even the wild house mice were related to great extent.The mouse probe generated a novel and highly individual specific mouse DNA fingerprint,compared to
human probe 33.6.

Key words: Parentage test, DNA fingerprinting, Mouse probe, Wild house mice

摘要: 本文以在英格兰捕获的野生小家鼠为研究材料。尝试用DNA指纹图谱方法判别动物后代的双亲.从冷冻鼠脑组织中提取的DNA,经限制性酶Hinf-I的酶切、凝胶电泳、尼龙膜吸附、与P 标记的人或鼠RNA探针杂交、放射自显影.最后得到DNA指纹图谱.图谱分析表明:野生小家鼠间的亲缘关系很近.个体间的相似性系数较高.不易判别后代的双亲,建议采用简便而又准确的条带比较法,取代相似性系数法:鼠探针与鼠DNA杂交所得到的图谱与人探针33.6的相比.个体的特异性更强.

关键词: 双亲判别, DNA指纹图谱, 鼠探针, 野生小家鼠