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STUDIES ON THE FINE STRUCTURE OF THEF0LLICLE CELLS IN THE DEVELOPMENT OF OOCYTES OF MINK

LIU Yutang, QIN Pengchun   

  • Online:2006-07-29 Published:2008-07-07

水貂卵母细胞发育过程中卵泡细胞的微细结构研究

刘玉堂, 秦鹏春   

  1. 东北林业大学野生动物资源学院.哈尔滨

Abstract: In this paper,formation of cumulus oophorus in mink(Mustela vison)were studied by light,electron microscope and histochemical techniques.Result showed that,during development of follicle cells,some round m itochondria with few cristae,numerous single rough endoplasmic reticulum cisternae and abundant free ribosomes present in the cytoplasm .few Golgi complex were seen.Stacks of microfilaments and som e vesicles(1-2um in diameter) that have a thin coat within it’s membrane occur as the follicle cell are unilaminar columnar.Exocytosis could be observed on the plasma membrane of follicle cells,and sometimes zona pellucida material is sandwiched in between these cells adjacent to the oocyte.It indicated that follicle cell might participate in formation of zona pellucida also.As the formation of the autrum ,the granules that contain phospholipids and neutral fat start to occur within follicle cells.During whole development,the follicle cell processes extend through the zona pellucida to anchor on the plasm membrane of the oocyte by means of intercellular attachments.In the later phase of the large antrum follicles,the corona radiate cells extend and make a obvious radial shape,opposing against the outer aspect of the zona pelucida.The extension appear in other cumulus cell in varying degrees too.Sheaves of microfilaments alternate with rough endoplasmic reticulum cistenae,and they run parallel to major axis within the extended cytoplasm of the corona radiate cells.It indicated that microfilaments play a great role in the extention of follicle cells.The extention of cumulus cells make whole cumulus oophorus turn into porous and filled with hyaluronic acid.It would be advantageous to sperm’s penetration and getting into oocyte afterwards.

Key words: Mink, Cumlus oophorus, Follicle cells, Morphological structure

摘要: 本文应用光镜,电镜及组织化学方法对水貂 (Mustela vison)卵丘形成过程进行了研究。结果表明,在发育过程中,卵泡细胞内含有一些圆形线粒体,许多单个粗面内质阿囊池和丰富的核糖体,高尔基复合体罕见。当卵泡细胞空成单层柱状时.细胞内开始出现成团的微丝及一些直径1—2 um的厚壁泡.在卵泡细胞膜上可见到胞吐小泡以及细胞间夹有透明带物质。表明卵泡细胞可能参与透明带的形成。随着卵泡腔的形成,卵泡细胞开始出现含中性脂肪及磷脂的颗粒。卵泡细胞始终通过胞质突起与卵母细胞保持接触。在大的有腔卵泡晚期,放射冠细胞发生伸展作用,形成明显的放射状结构,其它卵丘细胞也出现不同程度的伸展。在放射冠细胞伸展的胞质中可见许多微丝束及粗面内质网囊池相间平行排列,表明微丝的存在对卵泡细胞的伸展起着重要作用.卵丘细胞的扩展使整个卵丘细胞间隙加大充以透明质酸,这将有利于以后的精子穿过并进人卵母细胞中。

关键词: 水貂, 卵丘, 泡细胞, 形态结构, 组织化学