Previous Articles     Next Articles

ELECTRON MICROSCOPIC OBSERVATION AND LDH ISOENZYMES RESEARCH OF THE LIVER CELL IN NYCTALUS VELUTINUS

ZHANG Weidao, JIN Chengliu, ZHAO Ru, WANG Moqing   

  • Online:2006-07-29 Published:2008-07-07

绒山蝠肝细胞电镜观察和乳酸脱氢酶同工酶的研究

张维道,靳程留, 赵茹, 汪谟庆   

  1. 安徽师范大学生物系,芜湖

Abstract: Using light and electron microscopy techniques and polyacrylamide gel electrophoresis,we have studied the 1iver cells ultrastructure and its LDH isoenzyme in the Nyctalus velutinus.The results indicated that the abundant Mitochondria,rough endoplasmic reticulum,glycogen granules,ribosomes,lipid droplete,lysosomes and developed Golgi complex were present in the cytoplasm portions of the liver cells.There are a layer of rough endoplasmic reticulum to surround the mitochondria.This close relationship between the mitochondria and the rough endoplasmic reticulum (RER)were not observed in the other tissue ce1l of N.velutinus and a majority of mammals cells.LDH isoenzymes of the liver cell is essentially LDH-M ,that principally catalyze pyruvate to reduce into lactate.This indicates that energy production of mitochondria in the liver cell apparently not passed the path way of glycolysis→pyruvate→the oxidative phosphorylation of acetyl coenzyme A .but through degradation of fatty acid to offer the oxidative phosphorylation for acetyl coenzyme A.

Key words: Nyctalus velutinus, Liver cell, Mitochondria, LDH isoenzymes, Electron microscope

摘要: 本文用光镜和电镜观察了绒山蝠肝细胞的超微结构.用聚内烯酰胺凝胶电泳,对肝组织LDH同工酶进行了研究。肝细胞有丰富的线粒体、内质网、核糖体,发达的高尔基体、溶酶体等。在线粒体的周围有一层粗面内质网包绕,二者的密切关系,不仅在绒山蝠其他组织中,且在大部分哺乳动物细胞中均未观察到.诙种肝细胞的LDH 同工酶以M亚基为主.主要催化丙酮酸还原成乳酸。

关键词: 绒山蝠, 肝细胞, 线粒体, 电子显微镜, 乳酸脱氢酶同工酶