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STUDIES ON THE CHROMOSOMES OF YUNNAN HARE (LEPUS COMUS)

CHEN Zhiping, WANG Yingxiang, LIU Ruiqing   

  • Online:2006-07-29 Published:2007-09-13

云南兔(Lepus comus)的染色体研究

陈志平, 王应祥, 刘瑞清   

  1. 中国科学院昆明动物研究所

Abstract: The karyotype of Yunnan hare (Lepus coomus)has been studied by banding techniques (G-,C-banding)and silver staining. The number of diploid chromosome is 48,autosomes consist of 6 pairs of metacentrics, 5 pairs of submetacentrics, 10 pairs of subtelocentrics and 2 pairs acrobentrics. X is a larger submetaeentric.And very faint secondary constrictions is found on pairs 17,19.21.
The G-banded, C-banded and silver-stained karyotypes have been observed. The chromosomal homologies of genus Lepus are high by comparing their G-banded karyotypes.The certromeric heterochromatin is found in all chromosomes except Nos.9,14,l6, 20,whereas the centromeric C-bands of Nos.13, 15,18 are very fanitly stained.The sequential studies of conventional and silver stained karyotypes show Ag-NORs at the faint secondary constrictions of Nos. 17, 19,21. The Polymorphism of Ag-NORs of No.21 is found.And the karyotype evolution of Leporides has been discussed by comparison with other species.

Key words: Yunnan hare, Banding patterns of chromosomes, Karyotype evolution

摘要: 本文以染色体分带技术(G-,C-带和银染色),对云南兔(Lepus comus)的核型进行了观察分析。结果表明t:2n=48,NF=88。常染色体由6对中着丝粒染色体, 5对亚中着丝粒染色体,10对亚端着丝粒染色体和2对端着丝粒染色体组成;X染色体为亚中着丝粒染色体。除少数几对染色体(Nos.9,14,16,20)着丝粒C-带呈阴性外,其余均呈阳性或浅染,但异染色质的含量差异较大。Ag-NORs的数目为5-6个,分别位于Nos.1 7,19,21。通过与兔科其它属种核型的比较,对其染色体进化作了讨论。

关键词: 云南兔, 染色体带型, 核型进化