Abstract: Mitochondrial DNA (mtDNA ) samples from 9 individuals of Bandicota indica captured from 3 different sites in Guangdong province were digested individually with each of the following 13 restriction endonucleases Apa I、Bam H I、Bcl I、Bgl I、Cla I、Eco RI、Eco RV、Hind III、P st I、Pvu II、Sac I、Sca I and Xba I for RFLP analysis. All 31 fragements and 16 restriction morphs were recognized , the samples from differentsites were grouped into three mt DNA halotypes which could be distinguished by Pvu II ,Hind III and Apa I, indicating the mtDNA polymorphism with in B. indica . Restriction map was made by the double-enzyme digestion method and it would be very helpful to the study of the phylogentic relation ships among different species in the Murida. There were significant differences in RFLP of mtDNA among B. ind ica , rat and mouse, B. indica might have closer relationship with rat than that with mouse.

Key words: Bandicota indica, Mitochondrial DNA, Restriction map, RFLP (Restriction fragment length polymorphism)

摘要： 本文应用Apa I、Bam HI、Bcl I、Bgl I、Cla I、Eco RI、Eco RV、Hind III、Pst I、PvuII、Sac I、Sca I 和Xba I 等13种限制性内切酶对板齿鼠线粒体DNA (mtDNA ) 进行限制性片段长度多态(RFLP) 分析, 并用双酶解法构建其限制性内切酶图谱。结果表明板齿鼠存在3种mtDNA 单倍型, 可通过限制酶Pvu II、Hind III 和Apa I 区分, 呈现DNA 多态性和种内遗传变异。与小家鼠、褐家鼠mtDNA 限制性片段的数据相比较, 板齿鼠和这两种鼠mtDNA 存在明显差异。板齿鼠mtDNA 限制性内切酶图谱的建立, 为进一步系统研究鼠科动物的遗传分化提供了依据。

关键词: 板齿鼠, 线粒体DNA, 限制性内切酶图谱, DNA 多态性