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COMPARATIVE STUDIES OF C-BAND AND Ag-NOPs ON THE CHROM0S0MES BETWEEN TWO SPECIES GIBBONS

LIU Ruiqing,NIE Wenhui, CHEN   

  • Online:2006-07-25 Published:2008-07-07

两种长臂猿染色体的C带和Ag—NORs的比较研究

刘瑞清,佴文惠,陈玉泽,余定会   

  1. 中国科学晚昆明动物研究所,昆明

Abstract: This paper deals w ith the Cornparison of C-Banding patterns and Ag-NORs between white—browed gibbon (Hylobates hoolock)andwhite-cheeked gibbon(H.leucogenys). The results are given as below :The diploid number of H.hoolock is 38, and its karyotype formula is 18 (M or SM ), XY (M, dot). The white—checked gibbon has a diploid number of 2n= 52,and the karyotype formula is d 4 (M orSM ) + 6 (A ),XY (M ,A ).
There are com mon characteristics of C—banded patterns in H.hoolock and H.leucogenys, Which are centromeric distribution of heterochromatin ,loss of centromeric heterochromatin and incerstitiaI heterochromatin. There are alsO some diffefences among their C—banded paeterns,W hich are the C—banded a|so karyotype with constitutive heterochromatic arms only in H .hoolock,and with terminal heterochromatin only in H.leucogenys. Their the whole Y chromosomes are C -band positive·
Silver staining revealed one pair of SM chromosomes with NORs in H.hoolock.
There aro 5 chromosomes with Ag—NORs in male H.leucogenys,one of them is located on the Y chromosome which has been confirm ed by fluorescence in situs hybridization(FISH)in the same cell. The presence of an NOR on the Y Chromosome is unusual in mammals.T he female H.leucogenys has 4 chromosomes with Ag—N ORs.Moreover,the association of Ag—NORs has been observed in H.leucogenys. However, it has not been observed in H.hoolock.
Based on these results, the chromosomal evolution in gibbons has been discussed.

Key words: H.leucogenys, H.hoolock, Ag-NOR, C-Band

摘要: 本文对长臂猿属(Hylobates)有4个亚属,其中白眉长臂猿(H.hoolock)2n=38和白氟长臂猿(H.leucogenys)2n=52的染色体C带和Ag—NORs进行了比较研究。结果表明,两种长臂猿的C带核型中除多数染色休有着丝点C带外,一些染色体上还出现插入C带和着丝点C带弱化或
减少现象;白眉长臂猿有全异染色质臂;白颊长臂猿有较多的端位C带.白眉长臂猿有两个
Ag-NORs,而白颊长臂猿的Ag-NORs雌体有4个,雄体有5个,Y染色体上有NOR.本文对长臂猿的核型进化作了讨论。

关键词: 白眉长臂猿, 白颊长臂猿, C带, Ag-NORs