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STUDY ON SEMEN CHARACTER OF GIANT PANDA I.OBSERVATION OF SEMEN QUALITY

FENG Wen He, WANG Pengyan, WANG Chunrong, ZHANG Anju, YE Zhiyong, HE Guangxi   

  • Online:2006-07-26 Published:2007-09-14

大熊猫精液品质的研究I.精液质量的动态观察

冯文和, 王鹏彦, 王春蓉, 张安居, 叶志勇, 何光昕   

  1. 四川大学生物系

Abstract: Semen has been collected by electroejaculation from 41 occasions since 1979,andsemenbeing apt for artificial insemination can be cellected from 6- 20 year old male giant pandas in an interval of 7 days during oestrotus period. The fresh selden could be maintained for 61 hours in 0~4 degrees centigrade,the motility ( %) of the frozenthawed sprmatozoa preserved for 1~6 years is over30% after 90min. at 37 degrees centigrade.and that spermatozoa could get an effcct in artificial insemination.
Thesemen quality of giant panda is also compared with those of other animals of felidae and herbivorous.The high morphological abnormality rateof spermatozoa in giant panda isone of theose fatal reasons why the inseminationsuccess rate of the giant panda is low.So an important measure of improving the natural mating ability of the giant panda is to raise and administer the male animals scientifically.

Key words: Gian panda, Ailuropoda melanoleuca, Eleetroejaeuiation procedure, Semen vo]umes, Spe—rm motility, Semen check

摘要: 文用11 只大熊猫作了41次人工采精。其精液质量表明,在6-20岁的雄兽发情季节里,只要采精技术得当, 均能采到可用作人工授精的精液。鲜精在0—-4℃保存61小时,或冷冻精液超低温(-l96℃)保存1-6年,解冻后在37℃ 培养90分钟,其精子活力仍在3O%以上,用作人工授精,能达到受孕、产仔效果。 将大熊猫与几种猫科及食革动物的精液质量进行比较,大髂猫的精子畸形率(6-59 %,平均29.74 %)比其他动物高,这是大熊猫自然交配或人工授精后受孕率仍然很低的重要原因之一。对大熊猫进行科学饲养管理,可以提高大熊猫的性欲和自然交配能力。

关键词: 大熊猫, 电采精法, 精液, 精子活动, 精液检查