Abstract: By the method of isoelect ro-focusing PAGE, four kinds of isozymes in different tissues of Rattus niviventer captured in Beijing were studied. The dispersal and the activities of the isozyme bands were compared, and distinct organ specificity were found in EST isozymes, LDH isozymes and ADH isozymes, but not in SOD isozymes. The activities of isozymes were usually much higher in liver and kidney than in the other organs,but usually lower in spleen, lung and brain, except for EST isozymes in spleen and lung and except for LDH isozymes in brain. Heart muscle and skeleton muscle are significantly differential in LDH isozymes because of their difference in oxygen tension, nevertheless, they are quite similar in other three kinds of isozymes. The differences of various organs in isozymes are in keeping with their differences in physiological functions.

Key words: Rattus niviventer, LDH isozyme, ADH isozyme, EST isozyme, SOD isozyme, Tissue specificity

摘要： 用聚丙烯酰胺凝胶等电聚焦电泳方法, 分析了社鼠的肝、肾、心肌、骨骼肌、肺、脾和脑等多种组织器官的LDH、ADH、EST、和SOD 4种同工酶, 对各组织器官的酶带数目和分布,以及酶活性进行了比较研究。结果表明, 社鼠的LDH 同工酶、ADH 同工酶和EST 同工酶具有比较明显的组织特异性, 而SOD 同工酶的组织特异性较低。肺和脾除EST 同工酶活性较高外, 脑除LDH 同工酶活性较高外, 其它3种同工酶的活性均较低; 而肝和肾中4种同工酶的活性普遍很高。心肌和骨骼肌因氧张力不同而使LDH 同工酶酶谱存在明显差异, 但其它3种同工酶酶谱却非常相似。同工酶的组织特异性与各组织器官所执行的生理功能是相一致的

关键词: 社鼠, LDH 同工酶, ADH 同工酶, EST 同工酶, SOD 同工酶, 组织特异性