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NIU Hongxing,BU Yanzhen,YU Yan,JI Shengdong,WANG Yanmei,LU Quanwei,LIU Jingze
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牛红星, 卜艳珍, 余 燕, 姬生栋, 王艳梅, 卢全伟, 刘敬泽
Abstract: The kinds and activities of proteolytic enzymes in the digestive systems(tongue,esophagus,stomach,duodenum,liver,pancreas and large intestine) of two Amur tiger cubs (Panthera tigris altaica) were investigated using protein detection and SDS-G-PAGE. The results showed:1) the digestive system of Amur tiger cubs had 30 different kinds of proteolytic enzymes;2)the mostly optimal pH for these enzymes was a neutral environment,and an acid condition highly restrained their activity;3) the proteolytic enzymes in the tongue and esophagus were fewer than in other digestive organs and their activities were weak as well;4) the duodenum had the most kinds of proteolytic enzymes with the strongest activities under various pH conditions;5) the 16 kD enzyme bands always existed under neutral and alkalescence pH conditions in all digestive organs except the tongue.
Key words: Digestive system, Panthera tigris altaica, Proteolytic enzymes, SDS-G-PAGE
摘要:
关键词: 东北虎幼体, 消化系统, 蛋白水解酶, 复性电泳
NIU Hongxing,BU Yanzhen,YU Yan,JI Shengdong,WANG Yanmei,LU Quanwei,LIU Jingze. Preliminary studies on proteolytic enzymes in the digestive system of Amur tiger cubs(Panthera tigris altaica)[J]. .
牛红星, 卜艳珍, 余 燕, 姬生栋, 王艳梅, 卢全伟, 刘敬泽. 东北虎幼体消化系统蛋白水解酶的初步研究[J]. .
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http://www.mammal.cn/EN/Y2006/V26/I1/98