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Genetic variations of haemagglutinin protein gene of canine distemper virus isolated from breeding fox and raccoon dog

ZENG Xiangwei,LIU Lanlan,HUA Yuping   

  1. College of Wildlife Resources,Northeast Forestry University,Harbin 150040 ,China
  • Online:2008-09-04 Published:2008-07-07

圈养狐貉源犬瘟热病毒地方分离株H 基因的遗传变异分析

曾祥伟,刘澜澜,华育平   

  1. 东北林业大学野生动物资源学院,哈尔滨 150040

Abstract: Haemagglutinin (H) protein genes of six CDV strains isolated from fox and raccoon dog between 2004 and 2006 were amplified by RT-PCR, and then were cloned and sequenced. Their sequences were also compared with those of 34 CDV reference strains in Genbank. The results show that the ORF of H genes of six strains are all composed of 1 824 nucleotides, encoding a polypeptide of 607 amino acid residues. Deletion and insertion were not found in the H genes of the six isolates. Phylogenetic tree based on nucleotide sequences of H genes from six isolates and 34 reference strains show all these strains are classified into six majority genetic lineages, designated Asia - 1, Asia - 2, America - 1, America - 2, Europe, Arctic. HL isolate has high homology with Italy strain 194/97 and Danmark strain Green, and belongs to Arctic genetic lineage. The other five isolates have high homology with Japan strains HAMA,UENO,Tanu96 and China strains TN, GP, and belong to Asia -1 genetic lineage, this shows a certain extent geographical relativity. The six isolates have lower homology with vaccins strains Onderstepoor and Convac, it shows that virus variation may be one of important reasons for prevalence of CD in vaccinated animals.

Key words: Canine distemper virus, Genetic variation, Hemagglutinin protein gene

摘要: 本研究于2004 ~ 2006 年从人工饲养的发病狐貉中分离到6 个CDV 分离株,用RT - PCR 方法扩增了其H蛋白基因片段,并对其进行了克隆和测序。测序结果表明,6 个CDV 分离株H 基因阅读框全长均为1 824 bp,编码607 个氨基酸,未发现碱基的插入和缺失。与Genbank 中的34 株CDV 参考毒株的H 基因序列进行比较和分析,现有的CDV 毒株可以分为Asia - 1、Asia - 2 、America -1、America - 2、Europe 和Arctic 6 个基因型,本实验中的分离株HL 为Arctic 基因型,与来自意大利的194 /97 株、丹麦的Green 株同分在一组,其余5 株分离株与来自日本的HAMA、UENO、Tanu96 等毒株以及来自中国的TN 株、GP 株(大熊猫株)分在一组同属Asia -1型,表现出一定的地理位置相关性;6 个分离株均与Onderstepoor、Convac 等疫苗株差异较大、关系较远,提示病毒变异可能是造成目前已免疫动物仍然发生犬瘟热流行的原因之一。

关键词: 犬瘟热病毒, 血凝素蛋白基因, 遗传变异