Abstract: This study tested the effects of extracts of the climbing vine,Tripterygium wilfordii on the fertility of male Mongolian gerbils (Meriones unguiculatus). Based on pre-tests,three groups of animals were used to compare treatments:one group (n = 16)received an oral dose (40 mg/ kg)daily for 4 weeks,a second group (n = 10)received a single dose of 100 mg/ kg and a third,control group (n = 14)received the vehicle (1%CMC). All animals were autopsied 42 days after the start of treatment and the status of the testes and epdidymis were assessed. Animals treated with 40 mg / kg daily for 28 days showed significantly reduced fertility after mating trials (df = 1,P <0.05). Their testis and epididymis/ body weight ratios were significantly reduced as were the number and motility of their sperm which were also misshapen For the group receiving a single dose of 100 mg/ kg,the epididymis organ/ body weight ratio was significantly decreased and number of misshapen sperm was increased markedly,but no other parameters were different from the control group. These results indicate that continuous presentation of this plant extract over 4 weeks has a marked sterilizing effect on male Mongolian gerbils. A single treatment with a higher dose of the extract was not effective.

Key words: Antifertility activity Mongolian gerbils (Meriones unguiculatus) Preparations of Tripterygium wilfordii

摘要： 为了检测雷公藤制剂对长爪沙鼠繁殖功能的影响,根据预实验结果,将40 只雄性长爪沙鼠分为40 mg/ kg连续用药4 周组(n = 16)、100 mg / kg一次给药组(n = 10)和1% CMC 灌胃对照组(n = 14)处理,6 周后剖检,比较睾丸、附睾的脏器系数、精子密度、精子活力、精子形态和繁殖率等指标。结果表明40 mg / kg连续用药组鼠的睾丸、附睾脏器系数与对照组比较差异显著;精子密度、精子活力和活精子百分率显著下降;精子畸形率显著上升;繁殖率显著下降(df = 1,P < 0.05)。100 mg / kg一次给药组除附睾脏器系数显著下降、精子畸形率显著上升外,其它指标与对照组无明显差异。该药对长爪沙鼠连续作用4 周后不育效果明显,并且具有致畸性,在药物有效范围内,高浓度一次性给药方式不如较低浓度连续多次给药效果显著。

关键词: 不育作用 雷公藤制剂 长爪沙鼠