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The sterility effect of tripterygium glucosides (Tripterygium wilfordii) on male Brandt’s voles

LI Jimeng,ZHENG Min,GUO Yongwang,HAI Shuzhen,WU Xinping,JI Lili,SHI Dazhao   

  1. College of Agriculture and Biotechnology,China Agriculture University,Beijing 100084,China
  • Online:2009-03-08 Published:2009-03-08

雷公藤制剂对雄性布氏田鼠的不育作用

李季萌, 郑敏, 郭永旺, 海淑珍, 吴新平, 嵇莉莉, 施大钊   

  1. 中国农业大学农学与生物技术学院,北京 100094

Abstract: We tested the anti-fertility effects of Tripterygium wilfordii on adult male Brandt’s voles (Lasiopodomys
brandtii
) during the mating period. We tested two experimental protocols:allowing voles to ingest the preparation freely, and forcing ingestion continuously. We examined effects at 3 dosages:80mg/ kg,120mg/ kg and 160 mg/ kg. Dosages of 120 mg / kg and 160 mg / kg both caused testis organ coefficients to decrease,but the difference of dosage in groups was not significant (F(3,21) = 2.197,F 0.05 =3.07,P > 0.05). However,there was a dosage effect on the quantity and vigor of sperm in epididymis (F (3,21) = 17.305,F 0.05 = 3.07,P < 0.05), and the density and activity of sperm appeared to decline with an increase in dose. Sperm abnormalities also increased with dose. Doses of 80 mg/ kg and higher not only produced atrophy in the epididymis but also damaged testis organization. At a dose of 120 mg /kg,mean number of embryos and reproductive rates of Brandt’s voles were reduced. We documented no abnormalities among descendant offspring of treated voles. The optimal dosage of Tripterygium wilfordii was between 120 and 160 mg/ kg. We observed no significant
difference in effect between male voles allowed to ingest the treatment freely and voles forced continuously to ingest the treatment.

Key words: Antifertility control, Brandt's voles (Lasiopodomys brandtii), Tripterygium glucosides (Tripterygium wilfordii)

摘要: 采用强制给药和自由取食2 种方式,研究了雷公藤制剂对发育期成体雄性布氏田鼠不育作用,给药剂量分别为80 mg/ kg、120 mg /kg 和160 mg/ kg。结果表明,120 mg/ kg 和160 mg /kg 剂量使睾丸脏器系数下降,但剂量组间差异不明显(F(3,21) = 2.197,F 0.05 =3.07, P > 0.05)。不同剂量能够对附睾中精子数量和活力产生显著影响(F(3 ,21) = 17.305,F 0.05 = 3.07, P <0.05),且随药物剂量的升高精子密度与精子活力均呈下降趋势。精子畸形率亦随药物剂量的增加而增加。80 mg/ kg 及其更高的剂量使附睾出现萎缩并造成睾丸组织损伤。120 mg/ kg 剂量可减少布氏田鼠平均胎仔数及降低繁殖率。给药后的繁殖后代未发现畸形幼仔。雷公藤制剂的最佳使用剂量介于120 ~160 mg/ kg。自由取食与连续强制给药两种方式对雄性布氏田鼠的作用差异不显著。

关键词: 不育控制, 雷公藤制剂, 布氏田鼠