Abstract: A parvovirus strain isolated from the feces of blue foxes in Tai’an,It was identified was parvovirus by a series of physicochemical assays,viral hemagglutination test and by examining animals exposed to it. The primers were designed based on the VP1 genes of the strains of CPV and FPV in Genbank. The VP1 gene of the isolate was amplified by PCR, which was cloned into pMD18 - T vector,sequenced and analyzed. The ORF of the VP1 gene of the isolate included 2 256 bp and encoded 727 amino acids. The nucleotide homologies of VP1 genes between the isolate and the CPV,FPV references are from 98.7% to 99.5% . Phylogenetic analysis showed that the isolate and FPV had the smaller inheritance distances. The 375th amino acids of the VP1protein of the isolate were identical to those of CPV,but the 223th,236th, 246th,466th,707th and 711th amino acids of the VP1 protein were consistent with those of FPV. The sequence of the VP1 gene of the isolate suggested transition between FPV and CPV. It implied that the isolate was blue fox parvovirus,named blue fox parvovirus Taian isolate (BFPV - TA),and blue fox played an important role in CPV appearance.

Key words: Blue fox parvovirus, VP1 gene, Sequence analysis

摘要： 从泰安地区送检的疑是细小病毒感染的蓝狐粪便中分离到一株病毒。经理化特性鉴定、血凝谱鉴定、人
工感染蓝狐等鉴定,表明所分离病毒为细小病毒。并且根据GenBank 上发表的犬细小病毒(Canine parvovirus,
CPV)、猫细小病毒(Feline parvovirus,FPV)核酸序列,设计扩增VP1 基因的引物,采用PCR 技术扩增所分离
细小病毒的VP1 全基因,将PCR 产物克隆入pMD18 - T 载体,进行测序分析。结果,所分离细小病毒的VP1 基
因全长2 256 bp,编码727 个氨基酸,与CPV 和FPV 参照株的VP1 基因同源性在98. 7% ～ 99. 5% 。VP1 基因的
系统发生分析表明所分离病毒与FPV 的亲源关系最为密切。所分离病毒VP1 蛋白375 位氨基酸残基与CPV 的
VP1 蛋白氨基酸残基一致,但其223 位、236 位、246 位、466 位、707 位、711 位氨基酸残基与FPV VP1 蛋白的
氨基酸残基一致,该病毒VP1 蛋白序列表现出了过渡型序列特征,介于FPV 与CPV 间的过渡类型,这说明所分离病毒为蓝狐细小病毒(Blue fox parvovirus,BFPV),命名为BFPV - TA,蓝狐可能在CPV 的起源过程起到重要
的作用。

关键词: 蓝狐细小病毒, VP1 基因, 序列分析