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THE DISC ELECTROPHORESIS OBSERVATIONS ON LDH ISOENZYME OF TISSUES OF GIANT PANDA CARCASS

FENG Wenhe1, LUO Changrong1, HE Guangxin2, YE Ziyong2, CHANG Anju2   

  1. 1. Department of Biology, Sichuan University;
    2. Zoo of Chengdu
  • Online:2011-11-22 Published:2011-11-22

大熊猫尸体组织LDH同工酶盘电泳观察

冯文和1, 罗昌蓉1, 何光昕2, 叶志勇2, 张安居2   

  1. 1. 四川大学生物系;
    2. 成都动物园

Abstract: This paper deals with disc electrophoresis of LDH isoenzyme of 16 tissues of giant panda carcasses, the 16 tissues are cerebrum, cerebellum, spinal cord, crystal, bone marrow, skeletal muscle, cardiac muscle, gastric liquid, liver, spleen, kidney, adrenal gland, lung, ovary, testis and epididymis. 5 giant panda carcasses have been observed.The 16 tissues of giant panda carcasses have been studied by polyacryl-amide gel disc electrophoresis and the results are as follows:1. 5 components of LDH isoenzyme band of 16 tissues of giant panda carcasses range, from positive electrode to negative electrode, LDH1,LDH2, LDH3, LDH4, LDH5.2. The activity of LDH1 is higher, while the activities of LDH4 and LDH6 are lower in cerebrum, cerebellum, spinal cord, cardiac muscle, kidney, adrenal gland and epididymis; the activity of LDH5 is higher, while the activities of LDH1 and LDH2 are lower in gastric liquid, skeletal muscle and liver; the activity of LDH2 is higher, while the activities of LDH4 and LDH5 are lower in crystal, bone marrow, ovary and testis; the activity of LDH3 is higher, while the activity of LDH5 is lower in spleen and lung.

Key words: Giant panda, Polyacrylamide gel disc electrophoresis, Tissue organ, LDH isoenzyme, Biochemistry

摘要: 大熊猫是冰川时期残存至今的古老、稀有的珍贵动物,有“活化石”之称。对大熊猫的研究除野外调查(王朗自然保护区大熊猫调查组,1974)、形态解剖(张鹤宇等,1959;冯文和等,1984)、人工饲养繁殖(北京动物园,1974;冯文和等,1983;1984)等外,尚有生化技术和免疫学等研究方法(Sarich,1973;潘文石等,1982;罗昌容等,1984)。

关键词: 大熊猫, 聚丙烯酰胺凝胶盘电泳, 组织器官, LDH同工酶, 生物化学