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INVESTIGATION ON PREALBUMIN VALUE AND ANTITHROMBIN Ⅲ (AT-Ⅲ) MEASUREMENT IN RHESUS MONKEY SERUM

DING Zheng Liang1, ZHOU Huili1, HE Shuzhen2, HU Buoming3, ZHOU Kang3, FANG Guoding3, GU Xiuying3   

  1. 1. Department of Laboratory Animal, Shanghai Medical University;
    2. Shanghai Center of Laboratory Animals, Academia Sinica;
    3. Zhong Shan Hospital, Shanghai Medical University
  • Online:2011-11-23 Published:2011-11-22

恒河猴血清前白蛋白和抗凝血酶 Ⅲ火箭免疫电泳测定研究

丁正梁1, 邹惠莉1, 胡淑贞2, 贺伯明3, 周康3, 方国汀3, 顾秀英3   

  1. 1. 上海医科大学实验动物部;
    2. 中国科学院上海实验动物中心;
    3. 上海医科大学附属中山医院

Abstract: Prealbumin was measured in 67 Rhesus Monkeys (34 males and 33 females), weighed from 3 to 4 Kg and aged from 2 to 4 years. As a result, the value of prealbumin in Rhesus Monkeys serum was 64.9±14.5 mg/dl.Prealbumin is synthesized by liver and functions as a carrier of thyroxine and vitamin A. The prealbumin value may be referred to as a normal range. The method used was rocket electrophoresis, which is simple, rapid and accurate one to determine PA.Antithrombin (AT-Ⅲ) is an important component in anticoagulation system in blood as a natural inhibitor of thrombin. It's importance has been increasingly recognized.AT-Ⅲ was examined from 42 Rhesus Monkeys (21 males and 21 females), weighed from 3 to 4 Kg and aged from 2 to 4 years. As a result, AT-Ⅲ was 45.5±7.3 mg/dl (X±SD). Hence, The normal range was 33.3-61.7 mg/dl. The method used was rocket electrophoresis autography, Which is a more accurate method than thrombin neutralizing one.

Key words: Rhesus Monkeys, Macaca mulatta, Prealbumin, Antithrombin, Rocket electrophoresis

摘要: 本文应用火箭免疫电泳法测定了67只正常恒河猴血清中的前白蛋白和42只血清中抗凝血酶Ⅲ的参考值。 前白蛋白由肝脏合成能与甲状腺素及维生素A结合,与肝胆疾病有密切关系。抗凝血酶Ⅲ是生理性抗凝系统的主要成分。均在临床上被广泛应用。为了实验工作的需要,我们测定了恒河猴的这2项指标都比人类高,其原因可能与饲养条件、环境、营养等有关。

关键词: 恒河猴, 前白蛋白, 抗凝血酶Ⅲ, 火箭免疫电泳