|
|
|
| The application of HS-SPME-GC / MS in detecting chemical components in giant panda (Ailuropoda melanoleuca)urine |
| LIU Yuliang,XIN Qiuhong,LIU Dingzhen,CAI Zhigang,ZHANG Zhihe,LAN Jingchao,WEI Rongping,LI Desheng,HOU Rong |
| Chengdu Research Base of Giant Panda Breeding |
|
|
|
|
Abstract Urine plays an important role in chemical communication between giant pandas (Ailuropoda melanoleuca). The detection of chemical compounds in giant panda urine is the key to identifying the compositions and assaying the function of each chemical compound. We used headspace-solid phase microextraction (HS-SPME)and gas chromatography-mass spectrometry (GC/ MS)to pre-treat and analyze the composition of giant panda urine We found 56 peaks and through searching the mass spectrometry library of the National Institute of Standards and Technology (NIST),38 of them were putatively identified .In addition,we also modified the method of cleaning HS-SPME fiber.The results show that HS-SPME-GC / MS is an effective method to detect the volatile and semi-volatile compounds in giant panda urine. It is proved to be an important research tool for revealing the mechanism of chemical communication in the giant pandas.
|
|
|
|
|
|
| [1] |
MA Kai, WU Cunzhe, YIN Feng, LIU Xuefeng, ZHANG Jinguo, LIU Dingzhen. Effects of storage temperature and duration on cortisol concentration in feces of giant pandas (Ailuropoda melanoleuca)[J]. 兽类学报, 2014, 34(2): 188-192. |
| [2] |
ZHOU Hong,YUAN Shibin,YANG Zhisong,ZHENG Lisong,YANG Yi,GU Xiaodong,YANG Xuyu,ZHANG Zejun. Correlation between diet and biomass of staple-food bamboos utilized by giant pandas during summer in Liziping Nature Reserve,China[J]. , 2014, 34(1): 93-99. |
| [3] |
. Comparison of spatial positioning between radio telemetry (RT)and GPS in temperate mountain forests:A case study on tracking the reintroduction of captive giant pandas[J]. , 2012, 32(3): 193-202. |
| [4] |
. cDNA,genomic sequence cloning and overexpression of troponin C type(TNNC1)gene of the giant panda and the black bear[J]. , 2012, 32(3): 228-238. |
| [5] |
. The determination of blood physiological indices for the giant panda[J]. , 2012, 32(3): 266-270. |
| [6] |
. Effect of the Wenchuan earthquake on habitat use patterns of the giant panda:a case study of Longxi-Hongkou and Tangjiahe Nature Reserves[J]. , 2012, 32(2): 118-123. |
|
|
|
|
