Abstract November 2011,a two-year-old Baird’s tapir (Tapirus bairdii)suddenly died in the Beijing Zoo. This paper records the pathogen testing results. Cultures showed that the organs of Baird’s tapir did not contain any bacteria,but there were two main bacteria Clostridium perfringens and Streptococcus bovis,in the intestinal contents. The serotype of the C. perfringens was found to be type A by multiplex PCR method. Clinical characteristics and autopsy pathology ultimately determined that C. perfringens type A was the primary pathogen in the death of the Baird’s tapir,The role of S. bovis was minor.