ACTA THERIOLOGICA SINICA ›› 2015, Vol. 35 ›› Issue (2): 176-183.

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The effect of levonorgestrel-quinestrol on female fertility in Microtus fortis

ZHOU Xunjun, YANG Yuchao, WANG Yong, ZHANG Meiwen, LI Bo, PENG Zhen   

  1. Institute of Subtropical AgricultureChinese Academy of SciencesDongting Lake Station for Wetland Ecosystem ResearchCASKey Laboratory of Agro-ecological Processes in Subtropical Region
  • Online:2015-05-15 Published:2015-08-12
  • Contact: WANG Yong:wangy@isa.ac.cn

左炔诺孕酮和炔雌醚复合不育剂(EP-1)对雌性东方田鼠生殖的影响

周训军 杨玉超 王勇 张美文 李波 彭真


 
 
  

  1. 中国科学院亚热带农业生态研究所
  • 通讯作者: 王勇:wangy@isa.ac.cn
  • 作者简介:周训军(1986-)男,硕士,研究实习员,主要从事动物生态学和鼠类防治研究.
  • 基金资助:
    国家科技支撑资助项目(2012BAD19B02)

Abstract: Our laboratory study evaluated the effects of a contraceptive compound EP - 1 (levonorgestrel - quinestrol) on fertility in female Microtus fortis calamorum, as measured by changes in hormone levels, reproductive organs, litter size, pregnancy rate and reproduction interval. Some dosages of EP -1 significantly affected uterus coefficient, follicle - stimulating hormone (FSH)levels, ovary coefficient and ovarian tissue morphology, but didn’t obviously affect uterus length or estradiol levels. A dose of 60 mg/kg EP - 1 significantly increased FSH levels and ovary coefficients, however doses of 10 mg/kg or 30 mg/kg EP -1 had no obvious effect on either measurement. Litter size and pregnancy rate were significantly reduced at different dosages of EP -1. The effective sterilization periods following dosage with 10 mg/kg, 30 mg/kg or 60 mg/kg of EP -1 were approximately 16, 38 and 52 days respectively.The time taken for measurements to return to pre-dosing conditions was related to the dose of EP -1. Our results indicate further potential of EP -1 in reducing fertility for the control of Microtus fortis populations in field conditions.

Key words: EP -1 (Levonorgestrel - quinestrol), Female Microtus fortis, Fertility control

摘要:   本文在实验室条件下通过一次性灌胃的方式研究了左炔诺孕酮-炔雌醚复合不育剂(EP-1)对雌性东方田鼠激素、生殖系统、胎仔数、受孕率的影响及药效持续时间,旨在检验其应用于野外东方田鼠不育控制的价值。结果表明,各浓度EP-1灌胃均能显著提高雌性东方田鼠的子宫系数,使子宫形态发生变化,对卵巢组织造成损伤,影响卵泡和黄体的发育,但是对雌二醇含量和子宫长度没有明显影响;60mg/kg EP-1灌胃能显著提高促卵泡刺激素含量和卵巢系数,而10mg/kg、30mg/kg EP-1灌胃对二者没有明显影响。室内繁殖实验表明,各浓度EP-1灌胃均能不同程度延迟初次怀孕时间,并降低试鼠的怀孕率和胎仔数,但抗生育作用具有可逆性且与药物浓度相关,10mg/kg、30mg/kg和60mg/kg剂量组生殖系统恢复时间分别为16 d、38 d和52 d。

关键词: 雌性东方田鼠, EP-1, 不育控制