Abstract: To investigate the effects of cabergoline dosage and duration on the reproductive system of normal male animals, forty male rats were randomly divided into five groups and treated with cabergoline for 3 consecutive days at varying doses (0, 50μg/kg, and 100μg/kg)． Rats from T750 and T7100 were sacrificed by decapitation on 7 d after cabergoline administration, and those from T2450 and T24100 were sacrificed by decapitation on 24 d after cabergoline administration We then observed changes in testicular tissue cells and examined the activities of enzymes in testis such as acid phosphatase (ACP),alkaline phosphatase (AKP),lactate dehydrogenase (LDH)and glutathione peroxidase (GSH-Px)by colorimetry. Results showed that endogenous germ, stromal and supporting cells in seminiferous tubules were damaged, or even fell off and disintegrated. Cabergoline treatment at 100 μg/kg decreased the ACP enzyme activity,but it was restored on 24 d after cabergoline administration. Cabergoline did not affect AKP levels. The dose increased will decreased the amount of increasing of LDH enzyme activity, and recovery on 24 d after cabergoline treatment. The activity of GSH-Px was significantly increased at 24 d at 100 μg/kg cabergoline compared with the control group, and more than those in 50 μg/kg group. Hence,cabergoline has some influence on the reproductive system of males, and can significantly affect the activities of enzymes in the testis.

Key words: Acid phosphatase, Cabergoline, Lactate dehydrogenase, Rattus losea, Testis

摘要： 为探讨卡麦角林剂量和处理后时间对雄性黄毛鼠睾丸组织结构和相关酶活力的影响，以50μg/kg和100mg/kg卡麦角林连续3d灌胃雄鼠，在处理后第7d和24 d镜检睾丸组织并分析组织中酸性磷酸酶（Acid phosphatase，ACP）、碱性磷酸酶（Alkaline phosphatase，AKP）、乳酸脱氢酶（Lactate dehydrogenase，LDH）、谷胱甘肽过氧化物酶（Glutathione peroxidase，GSH-Px）的活力。结果发现：卡麦角林可使曲细精管内生精细胞、间质细胞和支持细胞受损，脱落解体。100µg/kg卡麦角林可降低ACP酶活力，处理后24 d时恢复；处理后AKP酶与对照无显著差异。处理组LDH酶活力显著增加，但剂量增加导致LDH活力增加幅度下降，且处理后24 d时恢复。100 µg/kg卡麦角林处理后24 d 时，GSH-Px酶活力显著高于对照组，且高于同浓度处理组第7d时的酶活力。处理后相同时间检测的GSH-Px活力高于50µg/kg处理组。结果表明卡麦角林对雄鼠生殖系统有一定的影响，可明显影响睾丸标志酶的活力。

关键词: 黄毛鼠, 卡麦角林, 睾丸, 酸性磷酸酶, 乳酸脱氢酶