Abstract The present study examined the effects of cold exposure on energy metabolism of striped hamsters, antioxidant activity and super-oxidative damage to organs or tissues, as well as the inter-relationships among the different organs and tissues. Body mass, food intake, superoxide dismutase (SOD) and catalase (CAT) activity, and H2O2 and malonadialdehyde (MDA) levels were measured in striped hamsters exposed to cold temperatures. Cold exposure increased food intake, but had no effect on body mass. MDA levels in heart and muscle, and SOD activity in skeletal muscle, were significantly increased in the hamsters exposed to cold for 42 days relative to those in their counterparts maintained at room temperature.The decreases in ambient temperature significantly decreses H2O2 levels of brain and kidneys, and SOD activity of liver and skeletal muscle, whereas increased MDA levels of brain, liver, lung and kidney, and SOD activity in brain and small intestine were significantly increased in the hamsters exposed to 8, 0 and -8 °C compared to those maintained at 21 °C. The correlations of antioxidant and super-oxidative radicals differed significantly among the different organs and tissues: the correlations within the same tissues were 100% in kidney, 66.7% in liver and 50.0% in muscle. These findings suggest that: 1) super-oxidative radical production is associated with the duration and gradient of cold exposure; 2) super-oxidative radical levels differ significantly among the different organs and tissues; 3) antioxidant activity is significantly correlated with super-oxidative radicals levels in some organs and tissues, which may be an important defensive system against the oxidative damages.