ACTA THERIOLOGICA SINICA ›› 2015, Vol. 35 ›› Issue (4): 438-444.

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Effects of cold exposure on energy regulation and hypothalamic neuropeptideexpression levels in Tupaia belangeri

ZHANG Hao, ZHENG Jia, ZHU Wanglong, LIU Jihong, SUN Shuran, GAO Wenrong, WANG Zhengkun   

  1. Key Laboratory of Ecological Adaptive Evolution and Conservation on Animals-Plants in Southwest Mountain Ecosystem of Yunnan Province Higher Institutes College, School of Life Science, Yunnan Normal University, Kunming 650500, China
  • Online:2015-11-13 Published:2015-11-15

冷驯化对中缅树鼩能量代谢和下丘脑神经肽表达量的影响

张浩, 郑佳, 朱万龙, 刘继红, 孙舒然, 高文荣, 王政昆   

  1. 云南省高校西南山地生态系统动植物生态适应进化及保护重点实验室,云南师范大学生命科学学院,昆明 650500
  • 通讯作者: 王政昆,Email:wangzk_123@163.com
  • 作者简介:张浩(1981- ),男,讲师,主要从事动物生理生态学研究.
  • 基金资助:
    国家国际科技合作专项项目(2014DFR31040)、十二五科技支撑项目(2014BAI01B00)、国家自然科学基金项目(No. 31360096)

Abstract: Tupaia belangeri is a species which is distributed in tropical and subtropical regions. In the present study, we tested the hypothesis that hypothalamic neuropetides were cues to induce adjustments in body mass regulation and metabolism in cold-exposed tree shrews (Tupaia belangeri)> Body mass, food intake and thermogenic capacity, serum leptin levels and hypothalamic neuropeptide expression levels were measured. The animals were subjected to either 5 for 28 days in cold-exposed, or maintained under 25 for 28 days. The results showed a significant increase in body mass and food intake in the cold exposure group, accompanied by a significant decrease in serum leptin levels, hypothalanic cocaine and amphetamine regulated transcript peptide (CART) mRNA and pro-opiomelanocortin (POMC) mRNA levels. Neuropeptide Y(NPY) mRNA was significantly increased in the cold exposure group, but there were no changes in hypothalamic agouti-related protein (AgRP) mRNA levels between the control and cold acclimation groups. All of the results suggest that hypoleptinemia partially contributes to cold-induced hyperphagia, which might involve the elevation of hypothalamic NPY, CART and POMC gene expression.

Key words: Cold acclimation, Energy regulation, Hypothalamic neuropeptide, Tupaia belangeri

摘要: 中缅树鼩是分布于热带地区的物种。为研究在寒冷环境中下丘脑神经肽对中缅树鼩生理适应性调节作用,本研究测定了经冷驯化处理后中缅树鼩的体重、摄食量、血清瘦素浓度以及下丘脑多种神经肽表达量的变化。结果显示:在冷驯化条件下,中缅树鼩体重和摄食量增加,血清瘦素浓度显著下降;神经肽Y(neuropeptide Y,NPY)表达量上升,可卡因-苯丙胺调节转录肽(cocaine and amphetamine regulated transcript peptide,CART)和阿片促黑色素原(pro-opiomelanocortin,POMC)表达量降低,豚鼠相关蛋白(agouti related peptide,AgRp)表达量无显著性差异。表明在冷驯化条件下,中缅树鼩主要通过改变NPY 、CART和POMC基因的表达水平等参与调节能量代谢。

关键词: 下丘脑神经肽, 冷驯化, 中缅树鼩, 能量调节