Abstract Eight adult Macaca fascicularis subjects (four males and four females) were collected between August 2013 to May 2014 and were divided into four same-sex groups. The intergroup differences of social status were determined via video, and a behavior study of feeding site preference and change was designed. Our results revealed that Macaca fascicularis subjects have significant feeding sit preference (75%), but it can be changed (87.5%). Exploratory behavior is closelyrelated to the change of feeding site preference. Male exploratory behaviors were more frequent than those of females, which may be associated with the closer proximity and relationships between females and males in the macaque colony structure. The exploratory behavior scores of high-status females were higher than those of low-status females. Therefore, gender and social status are important factors which can influence exploratory behavior. This study provided the evidence for pointing cues experiment, and new idea and direction for the research on brain functional asymmetry.