捕食风险应激对雄性布氏田鼠及子代繁殖性能的影响

doi:10.16829/j.slxb.150904

摘要/Abstract

摘要： 本研究旨在探讨雄性布氏田鼠暴露于捕食风险应激对自身及后代繁殖性能的影响，以及这种效应的阶段性。青春期是动物生长发育和对外界环境敏感的重要阶段，推测青春期所经历的捕食风险应激对雄鼠和后代繁殖性能的影响更为显著。实验选取90日龄成年期和28日龄青春期雄性布氏田鼠作为亲代，每天分别暴露于蒸馏水、兔尿和猫尿1 h，连续18 d。随后每组随机选取一半田鼠取样检测性腺器官指数、精子质量和数量等参数，另一半与正常雌鼠交配，待后代成年后检测繁殖性能。结果显示，与对照相比，成年期和青春期暴露于猫气味的雄性布氏田鼠精子正常率显著下降，精子畸形率和死亡率显著升高，而暴露于兔气味的雄鼠仅降低了正常精子比例；成年期雄性田鼠暴露于猫气味没有影响繁殖输出，而青春期暴露的雄鼠的后代在出生时雌性比例显著增加；成年期暴露于猫气味和兔气味都没有影响后代的繁殖性能，青春期暴露于猫气味的雄鼠的后代成年后性腺指数、精子密度和精子正常率降低，精子畸形率和死亡率显著升高，而暴露于兔气味仅降低了雄性后代的精子密度和精子正常率。以上结果验证了本研究假设，即捕食风险应激对布氏田鼠亲代和后代的繁殖性能均有一定的抑制作用，且青春期应激的效应更强更持久，存在明显的效应阶段依赖性。

关键词: 捕食风险, 父体效应, 繁殖性能, 布氏田鼠, 成年期, 青春期

Abstract: The present study aims to investigate whether predation risk stress can affect the reproductive characteristics of male Brandt’s voles (Lasiopodomys brandtii) and their offspring, and whether this is stage-specific. Adolescence is a crucial period for growth and development, and it is sensitive to external environmental factors. Thus, we speculated that exposure to predation risk stress during adolescence would have a more pronounced impact on the reproductive characteristics of both the males and their offspring. In this study, 90-day-old adult male Brandt’s voles and 28-day-old adolescent male Brandt’s voles were exposed to distilled water, rabbit urine, and cat urine for 1 hour daily for 18 consecutive days. Subsequently, half of each group was randomly selected for sampling to assess reproductive characteristics, including reproductive organ index, sperm quality and quantity. The remaining half was mated with a normal female, and their offspring's reproductive characteristics were evaluated until adulthood. The results indicated that, when compared to the control group, male Brandt’s voles exposed to cat odor during adulthood and adolescence significantly reduced normal sperm rate and increased sperm malformation and mortality rates, while exposure to rabbit odor only reduced the normal sperm rate. Exposure to cat odor during adulthood had no effect on reproductive output, whereas offspring from fathers exposed to cat odor during adolescence showed an increase in the ratio of female offspring at birth. Exposure to cat or rabbit odor during adulthood had no effect on offspring reproductive characteristics, but adult offspring from cat odor-exposed father during adolescence exhibited reduced gonadal index, sperm density, and normal sperm rate, as well as increased sperm deformity and mortality rates, while rabbit odor exposure only reduced sperm density and normal sperm rate in male offspring. These results confirmed our hypothesis that predation risk has a suppressive effect on the repro ductive characteristics of fathers and their offspring, especially during adolescence. The cross-generation transmission induced by paternal predation risk on offspring reproduction is stage-dependent.

Key words: Predation risk, Paternal effect, Reproductive characteristics, Brandt’s voles (Lasiopodomys brandtii), Adult hood, Adolescence

中图分类号:

Q958.12

周桃秀, 陈琳, 吴涌珍, 杨生妹, 魏万红, 武瑞勇. 捕食风险应激对雄性布氏田鼠及子代繁殖性能的影响[J]. 兽类学报, 2024, 44(5): 587-597.

ZHOU Taoxiu, CHEN Lin, WU Yongzhen, YANG Shengmei, WEI Wanhong, WU Ruiyong. Effects of predation risk stress on the reproductive characteristics of male Brandt’s voles and their offspring[J]. ACTA THERIOLOGICA SINICA, 2024, 44(5): 587-597.

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