关键词: 喜马拉雅旱獭, 聚丙烯酰胺凝胶盘电泳, 乳酸脱氢酶同工酶

Abstract: The distribution of the lactate dehydrogynase(LDH )isoenzyme bands in four tissues of the hibernation and non-hibernation himalayan marmor (Marmota himalayana)were analysed by means of the polyaczylamide ge1 disc electrophoresis and the oral LDH activity was determined． The results obtained are as follows：
1．Five bands were found in kidney and skeletal muscle，from anode to cathode in turn were LDH-1．LDH-2，LDH-3，LDH-4and LDH-5． Four bands were found in heart，andthe proper order were LDH -1，LDH-2，LDH-3 and LDH-4．Only 1iver tissues of hibernation differ from the ones of non-hibernation．two bands(LDH-4 and LDH -5)were discovered only in liver tissues of hihernation himalayan marmot．However，four bands，LDH-2，LDH-3，LDH-4 and LDH-5 Were found in liver of non-hibernation．Subbands appeared in the LDH isoenzymes of all other tissues except 1iver of hibernation himalayan marmot．
2． Tota1 enzyme activities of four tissues of hibernation ones were more or 1ess higher than the ones of non-hibernation．but there was no significant difference (P>0.05)，Only in skeletal muscle there was significance(P< 0.05)in terms of statistics．

Key words: Marmata himalayana, Polyacrylamide gel disc e1ectrophoresis, Lactate dehydrogenase isoenzyme