六种鼠苹果酸脱氢酶、血浆蛋白质和血红蛋白的变异分析

六种鼠苹果酸脱氢酶、血浆蛋白质和血红蛋白的变异分析

金晓玲，何新霞，周虞灿，吴林富，干常春

浙江师范大学生物系，金华

出版日期:2006-08-02 发布日期:2008-07-07

ANALYSES ON VARIATION OF MDH ， HEMOGLOBIN AND SOME PLASMA PROTEINS FOR SIX MURIDS

JIN Xiaoling , HE Xinxia , ZHOU Yucan , WU Linfu , GAN Changchun

Online:2006-08-02 Published:2008-07-07

摘要/Abstract

摘要： 本文分析了家鼠属（Rattus）4种鼠和姬鼠属（Apodemus）2种鼠的苹果酸脱氢酶、血浆中B₁区和B₂区蛋白质、血浆请蛋白、前清蛋白以及红细胞中血红蛋白的变异。结果表明，MDH在进化上是比较保守的，其中MDH_M和MDH_S2带在各鼠间位于同一泳动线上，仅MDH_S1带的泳动速度出现属间和种间的微小差异；血浆中B₁区和B₂区的蛋白质带、清蛋白带以及前清蛋白带各鼠间出现明显的差异，表现为黑线姬鼠和社鼠在B₁区出现2种多态型区带，褐家鼠在B₂区也有2种多态型带；各鼠间的血红蛋白变异比较明显，主要表现在区带数和主成分的泳动度显著不同，而且社鼠的Hb 出现3中多态型，黄毛鼠出现2种多态型，其与各鼠的Hb 皆为单态性。上述16项生化特性按相似和相异进行配对比较，初步表明黄毛鼠与褐家鼠亲缘上比较相近，白腹巨鼠要比其他3种鼠亲缘上更接近于黑线姬鼠，而社鼠与白腹巨鼠之间进化分歧相对的较大。

关键词: 生化分类, 鼠科动物, 血浆蛋白质, 血红蛋白, 苹果酸脱氢酶

Abstract: The MDH isozymes , hemoglobins and some plasma proteins of 4 species of the Rattus genus and 2species of the Apodemus genus were analysed by means of the vertical slab gel elect rophoresis . The results obtained showed that the MDH isozymes were rather conservative in evolueion . The MDH_M and MDH_S2 zones among all species analysed were respectively located to the same migratory line , but migratory speed of the MDH_S1 zones were different between the species of the two genus . The variations of migratory speed on the zones of albumin , pre –allbumin , the proteins in B₁and B₂ regions among the species were found . There were 2 polymorphic types in the B₁ region of A . agrarius and R . niviventer , respectively . There were also 2 polymorphic types in the B₂ region of R . norvegicus .It was also found to the variations of the migratory speed and the number of Hb zones among all species analysed . There were 2Hb polymorphic types for R. rattoid and 3 for R. niviventer . The phonetic analysis with 16 biochemical characters by the pair-group method showed that R . norvegicus was closed to R . rattoides in ally. The R . edwardsi was closer to A . agrarius than the res 3 species of the Rattus in ally . There were relatively marked divergence between R. edwardsi and R . niviventer in evolution .

Key words: Biochemical systematics, Murid, Plasma protein, Malate dehydrigenase(MDH), Hemoglobin(Hb)

金晓玲，何新霞，周虞灿，吴林富，干常春. 六种鼠苹果酸脱氢酶、血浆蛋白质和血红蛋白的变异分析[J]. .

JIN Xiaoling , HE Xinxia , ZHOU Yucan , WU Linfu , GAN Changchun. ANALYSES ON VARIATION OF MDH ， HEMOGLOBIN AND SOME PLASMA PROTEINS FOR SIX MURIDS[J]. .

[1]	韩春艳, 贾婷, 王炎, 王政昆, 朱万龙, 张浩. 冬季不同海拔大绒鼠MC1R基因的表达量及UCP1、Hb、Mb含量的比较[J]. 兽类学报, 2022, 42(1): 69-75.
[2]	傅必谦, 陈卫，高武，张可心，袁虹，芮朝胜，李举怀. 中华姬鼠与大林姬鼠的同工酶差异[J]. , 1998, 18(4): 304-310.
[3]	金晓玲，何新霞，周虞灿，杨君清，于立群. 六种鼠乳酸脱氢酶和超氧化物歧化酶位点变异的研究[J]. , 1998, 18(2): 144-149.
[4]	黄贵萍　黄钦皂　郭光红廖若男刘　菲. 野生大家鼠和实验大白鼠血红蛋白和乳酸脱氢酶同工酶的比较研究[J]. , 1997, 17(3): 216-220.
[5]	何新霞, 金晓玲, 周虞灿,郑祥建,　沈晖. 六种鼠组织中过氧化物酶和酯酶变异的比较[J]. , 1997, 17(2): 136-140.
[6]	石斌山, 诸葛阳. 臭鼩酯酶和苹果酸脱氢酶同工酶的电泳研究[J]. , 1993, 13(1): 25-30.
[7]	周虞灿, 邵邻相, 何新霞. 三种鼠科动物血浆超氧化物歧化酶和乳酸脱氢酶以及血浆蛋白成分的比较分析[J]. , 1990, 10(3): 231-234.
[8]	马莱龄、陈月仙、李绍文、李举怀. 秦岭地区大林姬鼠的酯酶和苹果酸脱氢酶同工酶的研究[J]. , 1989, 9(1): 63-67.
[9]	陆佩洪, 余多慰, 马玉银. 江豚MDH与ADH同工酶电泳研究[J]. , 1988, 8(2): 117-121.
[10]	周虞灿, 景增春, 温得启, 胡晓梅. 几种害鼠血红蛋白型的研究[J]. , 1985, 5(1): 17-23.