关键词: 牦牛, 乳腺上皮细胞, 细胞系, 生物学特性

Abstract: Abstract:The present study was carried out to establish a method of cultivation of yak mammary epithelial cell in vitro. Yak mammary epithelial cell line (YMEC)was established successfully by use of collagenase digestion,and then identified by immunocytochemistry,ultrastructural characteristics and RT-PCR. The morphological character,doubling time of cell population,growth curve,and chromosomal analysis of YMEC were characterized. The results showed that YMEC maintained a normal diploid chromosome modal number of 2n = 60,possessed an estimated population doubling time of 45 - 48 h and differentiation appeared after subculturing 25 times. The cell showed typical ‘cobblestone’morphology with numerous microvilli on its surface and there existed abundant amounts of rough endoplasmic reticulum and mitochondria in its cytoplasm. The result of sterility was negative. When the cells were stimulated by hormones,mRNA of beta-casein was detected. It was concluded that YMEC line retaining mammary gland specific functions was established,which might be a useful tool for studying the function of yak mammary epithelial cell.

Key words: Biological characteristics, Cell line, Mammary epithelial cells, Yak