关键词: 川金丝猴, 尿液, 睾酮, 孕酮, 雌二醇, 保存时效

Abstract: Analyzing steroid hormones in urine has been widely used in monitoring the physiology, health, and reproductive status of wildlife. Urine samples that are collected in the field must be stored before they are transferred and analyzed in the laboratory. Urinary steroid hormone metabolites vary across different species, necessitating different guidelines for storage duration and conditions. In this paper, 21 total urine samples from six adult golden snub-nosed monkeys (Rhinopithecus roxellana) were used to validate the standard sample storage duration. The results indicate that testosterone and progesterone from males at 4 ℃ can be stored up to 7 days, while estradiol from females can be stored for less than 3 days. Under -20 ℃, testosterone samples from males did not change significantly within 60 days. Testosterone, progesterone, and estradiol levels in the female samples did not change significantly within 60, 45, and 60 days, respectively. We conclude that keeping urine samples under -20 ℃ is an effective and reliable preservation method for the golden snub-nosed monkey.

Key words: Estradiol, Progesterone, Rhinopithecus roxellana, Testosterone, Urine