A hibernating male tube-nosed bat was collected in March, 2016 from Yanzi Cave in the Bamianshan Mountain (28°51′56″N, 109°16′15″E, 1213m asl), Longshan County, Xiangxi Tujia and Miao Nationalities Autonomous Perfecture, Hunan Province. This sample was identified as Murina fanjingshanensis based on both morphology and Cyt b gene sequence. The finding not only adds a new record of Chiroptera for Hunan Province, China, but also is the first report of the species outside its type locality in Fanjingshan Natural National Reserve, Guizhou, China.