半乳甘露聚糖检测在圈养海洋动物曲霉病早期诊断的应用

董贵信; 张德录; 黄泽林; 张孝敏; 梁淑余; 陈小芬; 沈博; 张鹏

doi:10.16829/j.slxb.150592

兽类学报 >

2022 , Vol. 42 >Issue 3: 339 - 344

DOI: https://doi.org/10.16829/j.slxb.150592

研究简报

半乳甘露聚糖检测在圈养海洋动物曲霉病早期诊断的应用

董贵信 ,
张德录 ,
黄泽林 ,
张孝敏 ,
梁淑余 ,
陈小芬 ,
沈博 ,
张鹏

展开

1. 广东长隆集团有限公司, 广州 511400;
2. 珠海长隆海洋王国, 珠海 519000;
3. 广东华南珍稀野生动物物种保护中心, 珠海 519031)

董贵信(1963-),男,博士,高级兽医师,广东长隆集团有限公司首席动植物官,主要从事野生动物保育与科普研究.

收稿日期: 2021-07-29

修回日期: 2022-02-23

网络出版日期: 2022-02-23

收起

Clinical application of galactomannan test in early diagnosis of aspergillosis in captive marine animals

DONG Guixin ,
ZHANG Delu ,
HUANG Zelin ,
ZHANG Xiaomin ,
LIANG Shuyu ,
CHEN Xiaofen ,
SHEN Bo ,
ZHANG Peng

Expand

1. Guangdong Chimelong Group, Co., Ltd., Guangzhou 511400, China;
2. Chimelong Ocean Kingdom, Zhuhai 519000, China;
3. Guangdong South China Rare Wild Animal Species Conservation Center, Zhuhai 519031, China

Received date: 2021-07-29

Revised date: 2022-02-23

Online published: 2022-02-23

Fold

本文引用格式

董贵信 , 张德录 , 黄泽林 , 张孝敏 , 梁淑余 , 陈小芬 , 沈博 , 张鹏 . 半乳甘露聚糖检测在圈养海洋动物曲霉病早期诊断的应用[J]. 兽类学报, 2022 , 42(3) : 339 -344 . DOI: 10.16829/j.slxb.150592

Abstract

Aspergillosis is one of the most common diseases in captive marine animals, and the early diagnosis of infection has not been reported. Galactomannan (GM) test is a good method for Aspergillus detection, which has a high clinical value in the early diagnosis of human medical Aspergillus infection, and can significantly improve the detection rate of Aspergillus patients. The purpose of this study was to investigate the clinical value of the GM test in the early diagnosis of aspergillosis infection in captive marine animals. The serum of 106 captive marine animals was tested for GM antigen, 25 were positive and 81 were negative. The dynamic changes of GM antigen levels before and after antifungal treatment were detected in 21 positive individuals. In addition, clinical data of the infected animals were collected for statistical analysis, and the value of GM detection for the early diagnosis of Aspergillus infection in captive marine animals was evaluated. When single I ≥ 0. 7 was used as the positive threshold for sea mammals and single I ≥ 1. 2 as the positive threshold for seabirds, the results showed that the sensitivity and specificity of the test were 91% for sea mammals and 94% for seabirds. This study shows that the GM test can effectively reflect the degree of Aspergillus infection in captive Marine animals through GM test, which can be an early, rapid and accurate diagnostic index, and has high clinical application value.

Key words： Galactomannan test; Aspergillosis; Marine animals; Early infection; Clinical application

参考文献

Fu Z M, Yu W J, Wang H Y, Wang Y P, Peng W D, Ying H J, Gu X. 2011. Utility of serum galactomannan in diagnosis and treatment of invasive fungal infection patients. Chinese Journal of Primary Care Medicine, 18 (19):2613-2614. (in Chinese)
Ge W, Liu Z M, Jiang C H, Hu H, Wang H Y. 2012. Evaluation of the galactomannan assays for the diagnosis of invasive pulmonary as pergillosis in the patients with respiratory disease. Chinese Jour nal of Tuberculosis and Defense, 34 (6):369-373. (in Chinese)
Geng Z, Ye C, Xiao Y, Wang D, Huang L, Li C R, Zhou J F. 2011. De tection of invasive aspergillosis by serum galactomannan test:A systematic review. Chinese Journal of Evidence-Based Medicine, 11 (9):1054-1061. (in Chinese)
Gulland F M D, Dierauf L A, Whitman K L. 2018. CRC Handbook of Marine Mammal Medicine. Third Edition. Los Angeles:CRC Press.
Infectious Diseases Society of America. 2016. Early diagnosis, effec tive therapy vital for treatment of deadly invasive aspergillosis.Clinical Infectious Diseases, June 30.
Itoh M, Toyotome T, Matsumoto N, Okamoto M, Yamada K. 2020.Characteristic imaging findings of the respiratory system in pen guins with suspected aspergillosis in an aquarium. Journal of Vet erinary Medical Science, 82 (9):1260-1266.
Ji Y, Liu D H, Xu L P, Liu K Y, Huang X J. 2007. The value of serum galactomannan detection for diagnosis of invasive aspergillosis in hematopoietic stem cell transplant recipients. Chinese Journal of Hematology, 28 (2):83-86. (in Chinese)
Johan M, Johan V E, Jan V, Erik V, Johny V, Marc B. 2002. Use of circulating galactomannan screening for early diagnosis of inva sive aspergillosis in allogeneic stem cell transplant recipients.The Journal of Infectious Diseases, 186 (9):1297-1306.
Lin S G, Lin Q Y, Chen G H, Guo J B, Lin W H. 2020. The diagnostic value of GM and G tests for mechanical ventilation complicated by pulmonary Aspergillus infection. Journal of Putian University, 27 (5):49-53. (in Chinese)
Liu Y D, Zhu J, Yang Y N, Wei J, Wei D L, Gao Y R, Wan L P, Wang C. 2014. Diagnostic value of GM test of invasive aspergillosis in patients with hematologic disease. Progress in Modern Biomedi cine, 14 (32):6315-6320. (in Chinese)
Maertens J A, Klont R, Masson C, Theunissen K, Meersseman W, La grou K, Heinen C, Crepin B, Van Eldere J, Tabouret M, Donnelly J P, Verweij P E. 2007. Optimization of the cutoff value for the a spergillus double-sandwich enzyme immunoassay. Clinical Infec tious Diseases, 44 (10):1329-1336.
Marcio N, Marr K A. 2005. Emerging fungi disease.Clinical Infec tious Diseases, 41 (4):521-526.
Marr K A, Balajee S A, McLaughlin L, Tabouret M, Bentsen C, Walsh T J. 2004. Detection of galactomannan antigenemia by enzyme immunoassay for the diagnosis of invasive aspergillosis:variables that affect performance. The Journal of Infectious Diseases, 190(3):641-649.
Melo A M, SilvaFilho R, Poester V R, Fernandes C G, Groll A V, Ste vens D A, Sabino R, Xavier M O. 2020. Aspergillosis in alba trosses. Medical Mycology, 58:852-855.
MenninkKersten M A, Donnelly J P, Verweij P E. 2004. Detection of circulating galaetomannan antigenemia for the diagnosis and man agement of invasive aspergillosis.Lancet Infectious Diseases, 4(6):349-357.
Schneider T, Olsen D, Dykstra C, Huettner S, Branch S, McClements R, Urquhart H. 2014. Penguin (Spheniscidae) Care Manual. The Association of Zoos and Aquariums in association with the AZA Animal Welfare Committee.
Seyedmousavi S, Guillot J, Arné P, de Hoog G S, Mouton J W, Melchers W J G, Verweij P E. 2015. Aspergillus and aspergillo ses in wild and domestic animals:a global health concern with parallels to human disease. Medical Mycology, 53:765-797.
Wang Z X. 2010. Classification of anti-fungal medicine andits func tional mechanism. Chinese Journal of Practical Pediatrics, 25(8):592-596. (in Chinese)
Xing Y L. 2011. The study on early diagnosis of invasive aspergillosis by the use of galactomannan assay and real-time fluorescent quan titative PCR detection. Master thesis. Guangzhou:Sun Yat-sen University.
Zhao B L, Wen Y T, Lv P H, Shi Y. 2007. Evaluation of the galacto mannan assay for the diagnosis of invasive aspergillosis in an ani mal model. Chinese Journal of Tuberculosis and Respiratory Dis eases, 30 (11):839-843. (in Chinese)
中华医学会重症医学分会. 2007. 重症患者侵袭性真菌感染诊断与治疗指南 (2007). 中华内科杂志, 46 (11):960-966.
中华内科杂志编辑委员会.2007. 血液病/恶性肿瘤患者侵袭性真菌感染的诊断标准与治疗原则 (修订版).中华内科杂志, 46(7):607-610.
王朝霞. 2010. 抗真菌药物的分类及其作用机制. 中国实用儿科杂志, 25 (8):592-596.
卢洪洲, 钱雪琴, 徐和平. 2018. 医学真菌检验与图解. 上海:上海科学技术出版社.
卢婷, 王子璠, 艾尼瓦尔·吾拉木, 宋玉霞. 2021. G试验联合GM试验对HIV合并侵袭性真菌感染的诊断效能分析. 世界最新医学信息文摘, 21 (48):252-253.
纪宇, 刘代红, 许兰平, 刘开彦, 黄晓军. 2007. 血清半乳甘露聚糖检测对造血干细胞移植后患者侵袭性曲霉菌感染的诊断价值.
中华血液学杂志, 28 (1):83-86.
李世荣, 王红, 张淑文. 2010. 半乳甘露聚糖对侵袭性曲霉菌感染的诊断价值. 中国真菌学杂志, 5 (6):381-384.
刘亚迪, 朱骏, 杨一宁,卫菊, 魏道林, 高彦荣, 万理萍, 王椿. 2014.
GM 抗原检测对血液病患者侵袭性曲霉病的诊断价值. 现代生物医学进展, 14 (32):6315-6320.
刘正印, 王爱夏. 2007. 侵袭性真菌感染的若干问题. 内科危重症杂志, 13 (2):98-100.
邢艳利. 2011. 半乳甘露聚糖检测及实时荧光定量PCR在侵袭性肺曲霉菌病早期诊断价值的实验研究. 广州:中山大学学位论文.
林少刚, 林群英, 陈国欢, 郭建斌, 林伟华. 2020. GM, G试验对机械通气并发肺曲霉菌感染的诊断价值. 莆田学院学报, 27 (5):49-53.
赵蓓蕾, 文昱婷, 吕沛华, 施毅. 2007. 血清半乳甘露聚糖检测诊断侵袭性肺曲霉病的实验研究. 中华结核和呼吸杂志, 30 (11):839-843.
耿哲, 叶丛, 肖毅, 王迪, 黄亮, 李春蕊, 周剑峰. 2011. 国内ELISA血清半乳甘露聚糖检测诊断侵袭性曲霉菌病价值的系统评价.
中国循证医学杂志, 11 (9):1054-1061.
傅中明, 俞万钧, 王华英, 汪一萍, 彭卫东, 应华娟, 顾霄. 2011. 血清半乳甘露聚糖检测对真菌感染的诊治价值. 中国基层医药, 18(19):2613-2614
葛伟, 刘志敏, 蒋彩花, 胡华, 王海英. 2012.血清半乳甘露聚糖检测对呼吸系统疾病患者侵袭性肺曲霉病的诊断价值.中国防痨杂志, 34 (6):369-373.

Options

文章导航

模态框（Modal）标题

本文引用格式

Abstract

参考文献