非人灵长类动物种质冷冻保存研究进展和展望
收稿日期: 2021-10-22
录用日期: 2022-03-14
网络出版日期: 2022-12-02
Advances and prospects in germplasm preservation of non-human primates
Received date: 2021-10-22
Accepted date: 2022-03-14
Online published: 2022-12-02
李明文 . 非人灵长类动物种质冷冻保存研究进展和展望[J]. 兽类学报, 2022 , 42(6) : 728 -740 . DOI: 10.16829/j.slxb.150626
Non-human primates are an important part of the biodiversity and valuable laboratory animals for biomedical research, however, wild populations of many non-human primate species have declined alarmingly, even on the brink of extinction, due to human activities, habitat destruction, hunting, and genetic isolation. Germplasm preservation is of great significance to the rescue of non-human primates and preservation of genetic materials. This article reviews research progresses in cryopreservation of sperm, oocytes, embryos, and gonadal tissues in the groups of New World monkeys, Old World monkeys, and great apes, and introduces the main methods of germplasm preservation in marmosets, squirrel monkeys, rhesus monkey, cynomolgus monkeys, and chimpanzees. The future research directions of non-human primate germplasm preservation are also discussed.
Key words： Non-human primates; Cryopreservation; Sperm; Oocyte; Embryo; Gonadal tissues
| null | Amidi F, Pazhohan A, Shabani N M, Khodarahmian M, Nekoonam S. 2016. The role of antioxidants in sperm freezing: a review. Cell Tissue Bank, 17 (4): 745-756. |
| null | Amorim C A, Jacobs S, Devireddy R V, Van Langendonckt A, Vanacker J, Jaeger J, Luyckx V, Donnez J, Dolmans M M. 2013. Successful vitrification and autografting of baboon (Papio anubis) ovarian tissue. Human Reproduction, 28 (8): 2146-2156. |
| null | Amorim C A, Donnez J, Dehoux J P, Scalercio S R, Squifflet J, Dolmans M M. 2019. Long?term follow?up of vitrified and autografted baboon (Papio anubis) ovarian tissue. Human Reproduction, 34 (2): 323-334. |
| null | Arakaki P R, Nichi M, Monteiro F O B, Muniz J A P C, Guimar?es M A B V, Valle R D. 2019a. Comparison of semen characteristics and sperm cryopreservation in common marmoset (Callithrix jacchus) and black?tufted?ear marmoset (Callithrix penicillata). Journal of Medical Primatology, 48 (1): 32-42. |
| null | Arakaki P R, Salgado P A B, Losano J D A, Gon?alves D R, Valle R D, Pereira R J G, Nichi M. 2019b. Semen cryopreservation in golden?headed lion tamarin, Leontopithecus chrysomelas . American Journal of Primatology, 81 (12): e23071. |
| null | Aslam H, Schneiders A, Perret M, Weinbauer G F, Hodges J K. 2002.Quantitative assessment of testicular germ cell production and kinematic and morphometric parameters of ejaculated spermatozoa in the grey mouse lemur, Microcebus murinus . Reproduction, 123 (2): 323-332. |
| null | Baert Y, Onofre J, Van Saen D, Goossen G. 2018. Cryopreservation of human testicular tissue by isopropyl-controlled slow freezing. Methods in Molecular Biology, 1748: 287-294. |
| null | Balmaceda J P, Heitman T O, Garcia M R, Pauerstein C J, Pool T B. 1986. Embryo cryopreservation in cynomolgus monkeys. Fertility and Sterility, 45 (3): 403-406. |
| null | Bhat G R, Sofi K A. 2021. Oocyte and embryo preservation in wild animals: an update. Cryo Letters, 42 (5): 251-260. |
| null | Brun B, Rumpler Y. 1990. Seasonal variation of sperm morphology in the Mayonette brown lemur (Eulemur fulvus mayottensis). Folia Primatologica (Basel), 55 (1): 51-56. |
| null | Cankut S, Dinc T, Cincik M, Ozturk G, Selam B. 2019. Evaluation of sperm DNA fragmentation via halosperm technique and TUNEL assay before and after cryopreservation. Reproductive Sciences, 26 (12): 1575-1581. |
| null | Chang M C, Hild S, Grieder F. 2021. Nonhuman primate models for SARS-CoV-2 research: Consider alternatives to macaques. Lab Animal (NY), 50 (5): 113-114. |
| null | Chatfield J, Penfold L. 2007. Prevention of urethral blockage following semen collection in two species of lemur, Varecia variegata variegata and Lemur catta . Journal of Zoo and Wildlife Medicine, 38 (2): 280-284. |
| null | Chatterjee S, Gagnon C. 2001. Production of reactive oxygen species by spermatozoa undergoing cooling, freezing, and thawing. Molecular Reproduction and Development, 59 (4): 451-458. |
| null | Chen B B, Li S S, Yan Y P, Duan Y C, Chang S H, Wang H, Ji W Z, Wu X, Si W. 2017. Cryopreservation of cynomolgus macaque (Macaca fascicularis) sperm with glycerol and ethylene glycol, and its effect on sperm-specific ion channels-CatSper and Hv1. Theriogenology, 104: 37-42. |
| null | Comizzoli P. 2017. Biobanking and fertility preservation for rare and endangered species. Animal Reproduction, 14 (1): 30-33. |
| null | Curnow E C, Kuleshova L L, Shaw J M, Hayes E S. 2002. Comparison of slow? and rapid?cooling protocols for early-cleavage-stage Macaca fascicularis embryos. American Journal of Primatology, 58 (4): 169-174. |
| null | de Carvalho F M, Ramsey C, Hanna C B, do Valle R D R, Nichi M, Binelli M, Guimar?es M A B V, Hennebold J D. 2021. Cryopreservation and preparation of thawed spermatozoa from rhesus macaques (Macaca mulatta) for in vitro fertilization. Journal of the American Association for Laboratory Animal Science, 60 (4): 396-406. |
| null | Devi L, Goel S. 2016. Fertility preservation through gonadal cryopreservation. Reproductive Medicine and Biology, 15 (4): 235-251. |
| null | Dolmans M M, Binda M M, Jacobs S, Dehoux J P, Squifflet J L, Ambroise J, Donnez J, Amorim C A. 2015. Impact of the cryopreservation technique and vascular bed on ovarian tissue transplantation in cynomolgus monkeys. Journal of Assisted Reproduction and Genetics, 32 (8): 1251-1262. |
| null | Dolmans M M, Donnez J, Cacciottola L. 2021. Fertility preservation: The challenge of freezing and transplanting ovarian tissue. Trends in Molecular Medicine, 27 (8): 777-791. |
| null | Dong Q, Correa L M, VandeVoort C A. 2009. Rhesus monkey sperm cryopreservation with TEST-yolk extender in the absence of permeable cryoprotectant. Cryobiology, 58 (1): 20-27. |
| null | Dong Q, Tollner T L, Rodenburg S E, Hill D L, VandeVoort C A. 2010. Antioxidants, Oxyrase, and mitochondrial uncoupler 2,4-dinitrophenol improved postthaw survival of rhesus monkey sperm from ejaculates with low cryosurvival. Fertility and Sterility, 94 (6): 2359-2361. |
| null | Dong Q, VandeVoort C A. 2009. Effect of egg yolk on cryopreservation of rhesus monkey ejaculated and epididymal sperm. Journal of Andrology, 30 (3): 309-316. |
| null | Dong Q X, Rodenburg S E, Hill D, Vandevoort C A. 2011. The role of low?density lipoprotein (LDL) and high?density lipoprotein (HDL) in comparison with whole egg yolk for sperm cryopreservation in rhesus monkeys. Asian Journal of Andrology, 13 (3): 459-464. |
| null | Fahy G M, Wowk B. 2021. Principles of ice?free cryopreservation by vitrification. Methods in Molecular Biology, 2180: 27-97. |
| null | Fayomi A P, Peters K, Sukhwani M, Valli?Pulaski H, Shetty G, Meistrich M L, Houser L, Robertson N, Roberts V, Ramsey C, Hanna C, Hennebold J D, Dobrinski I, Orwig K E. 2019. Autologous grafting of cryopreserved prepubertal rhesus testis produces sperm and offspring. Science, 363 (6433): 1314-1319. |
| null | Gadea J, Molla M, Selles E, Marco M A, Garcia?Vazquez F A, Gardon J C. 2011. Reduced glutathione content in human sperm is decreased after cryopreservation: Effect of the addition of reduced glutathione to the freezing and thawing extenders. Cryobiology, 62 (1): 40-46. |
| null | Gadea J, Toledano?Díaz A, Navarro?Serna S, Jiménez?Movilla M, Soriano P, Matás C, Santiago?Moreno J. 2019. Assessment and preservation of liquid and frozen-thawed black crested mangabey (Lophocebus aterrimus) spermatozoa obtained by transrectal ultrasonic?guided massage of the accessory sex glands and electroejaculation. Animal Reproduction Science, 210: 106176. |
| null | Gould K G, Styperek R P. 1989. Improved methods for freeze preservation of chimpanzee sperm. American Journal of Primatology, 18 (4): 275-284. |
| null | Hermann B P, Sukhwani M, Winkler F, Pascarella J N, Peters K A, Sheng Y, Valli H, Rodriguez M, Ezzelarab M, Dargo G, Peterson K, Masterson K, Ramsey C, Ward T, Lienesch M, Volk A, Cooper D K, Thomson A W, Kiss J E, Penedo M C, Schatten G P, Mitalipov S, Orwig K E. 2012. Spermatogonial stem cell transplantation into rhesus testes regenerates spermatogenesis producing functional sperm. Cell Stem Cell, 11 (5): 715-726. |
| null | Hildebrandt T B, Hermes R, Goeritz F, Appeltant R, Colleoni S, de Mori B, Diecke S, Drukker M, Galli C, Hayashi K, Lazzari G, Loi P, Payne J, Renfree M, Seet S, Stejskal J, Swegen A, Williams S A, Zainuddin Z Z, Holtze S. 2021. The ART of bringing extinction to a freeze-history and future of species conservation, exemplified by rhinos. Theriogenology, 169: 76-88. |
| null | Honaramooz A, Li M W, Penedo M C, Meyers S, Dobrinski I. 2004. Accelerated maturation of primate testis by xenografting into mice. Biology of Reproduction, 70 (5): 1500-1503. |
| null | Huleihel M, Lunenfeld E. 2020.Approaches and technologies in male fertility preservation. International Journal of Molecular Sciences, 21 (15): 5471. |
| null | Ishibashi H, Motohashi H H, Kumon M, Yamamoto K, Okada H, Okada T, Seki K. 2013. Ultrasound?guided non?surgical embryo collection in the common marmoset. Reproductive Biology, 13 (2): 139-144. |
| null | Jahnukainen K, Ehmcke J, Hergenrother S D, Schlatt S. 2007. Effect of cold storage and cryopreservation of immature non?human primate testicular tissue on spermatogonial stem cell potential in xenografts. Human Reproduction, 22 (4): 1060-1067. |
| null | Jung S E, Ahn J S, Kim Y H, Kim B J, Won J H, Ryu B Y. 2020. Effective cryopreservation protocol for preservation of male primate (Macaca fascicularis) prepubertal fertility. Reproductive BioMedicine Online, 41 (6): 1070-1083. |
| null | Kanda A, Nobukiyo A, Yoshioka M, Hatakeyama T, Sotomaru Y. 2018. Quality of common marmoset (Callithrix jacchus) oocytes collected after ovarian stimulation. Theriogenology, 106: 221-226. |
| null | Karimfar M H, Niazvand F, Haghani K, Ghafourian S, Shirazi R, Bakhtiyari S. 2015. The protective effects of melatonin against cryopreservation?induced oxidative stress in human sperm. International Journal of Immunopathology and Pharmacology, 28 (1): 69-76. |
| null | Kinoshita K, Indo Y, Tajima T, Kuze N, Miyakawa E, Kobayashi T, Nakamura T, Ogata M, Okumura F, Hayakawa T, Morimura N, Mori Y, Okamoto M, Ozaki Y, Hirata S. 2021. Comparative analysis of sperm motility in liquid and seminal coagulum portions between Bornean orangutan (Pongo pygmaeus) and chimpanzee (Pan troglodytes). Primates, 62 (3): 467-473. |
| null | Kometas M, Christman G M, Kramer J, Rhoton?Vlasak A. 2021. Methods of ovarian tissue cryopreservation: Is vitrification superior to slow freezing?-Ovarian tissue freezing methods. Reproductive Sciences, 28 (12): 3291-3302. |
| null | Kropp J, Di Marzo A, Golos T. 2017. Assisted reproductive technologies in the common marmoset: an integral species for developing nonhuman primate models of human diseases. Biology of Reproduction, 96 (2): 277-287. |
| null | Kuwayama M, Vajta G, Kato O, Leibo S P. 2005. Highly efficient vitrification method for cryopreservation of human oocytes. Reproductive BioMedicine Online, 11 (3): 300-308. |
| null | Ladanyi C, Mor A, Christianson M S, Dhillon N, Segars J H.2017. Recent advances in the field of ovarian tissue cryopreservation and opportunities for research. Journal of Assisted Reproduction and Genetics, 34 (6): 709-722. |
| null | Le?o D L, Miranda S A, Brito A B, Lima J S, Santos R R, Domingues S F. 2015. Efficacious long?term cooling and freezing of Sapajus apella semen in ACP-118. Animal Reproduction Science, 159: 118-123. |
| null | Lee D M, Yeoman R R, Battaglia D E, Stouffer R L, Zelinski?Wooten M B, Fanton J W, Wolf D P. 2004. Live birth after ovarian tissue transplant. Nature, 428 (6979): 137-138. |
| null | Lee D M, Thomas C M, Xu F, Yeoman R R, Xu J, Stouffer R L, Wolf D P, Zelinski M B.2017. Subcutaneous ovarian tissue transplantation in nonhuman primates: duration of endocrine function and normalcy of subsequent offspring as demonstrated by reproductive competence, oocyte production, and telomere length. Journal of Assisted Reproduction and Genetics, 34 (11): 1427-1434. |
| null | Li M W, Lloyd K C K. 2020. DNA fragmentation index (DFI) as a measure of sperm quality and fertility in mice. Scientific Reports, 10 (1): 3833. |
| null | Li M W, Meyers S, Tollner T L, Overstreet J W. 2007. Damage to chromosomes and DNA of rhesus monkey sperm following cryopreservation. Journal of Andrology, 28 (4): 493-501. |
| null | Li M W, Vallelunga J M, Kinchen K L, Rink K L, Zarrabi J, Shamamian A O, Lloyd K C. 2014. IVF recovery of mutant mouse lines using sperm cryopreserved with MTG in cryovials. Cryo Letters, 35 (2): 145-153. |
| null | Li X Y, Yao X Y, Mu C L, Wang Z X, Hu R, Chang Y, Wu J. 2022. Serum? and feeder?free culture of juvenile monkey female germline stem cells and testosterone regulation of their self?renewal. Stem Cell Reviews and Reports, 18 (1): 336-345. |
| null | Li Y H, Cai K J, Su L, Guan M, He X C, Wang H, Kovacs A, Ji W Z. 2005. Cryopreservation of cynomolgus monkey (Macaca fascicularis) spermatozoa in a chemically defined extender. Asian Journal of Andrology, 7 (2): 139-144. |
| null | Lopes F, Pinto?Pinho P, Gaiv?o I, Martins?Bessa A, Gomes Z, Moutinho O, Oliveira M M, Peixoto F, Pinto?Leite R. 2021. Sperm DNA damage and seminal antioxidant activity in subfertile men. Andrologia, 53 (5): e14027. |
| null | Luvoni G C, Colombo M. 2020. Cold case: small animal gametes cryobanking. Theriogenology, 150: 445-451. |
| null | Mazur P, Leibo S P, Seidel G E Jr. 2008. Cryopreservation of the germplasm of animals used in biological and medical research: importance, impact, status, and future directions. Biology of Reproduction, 78 (1): 2-12. |
| null | McCarthy M J, Meyers S A. 2011. Antioxidant treatment in the absence of exogenous lipids and proteins protects rhesus macaque sperm from cryopreservation-induced cell membrane damage. Theriogenology, 76 (1): 168-176. |
| null | Moraveji S F, Esfandiari F, Sharbatoghli M, Taleahmad S, Nikeghbalian S, Shahverdi A, Baharvand H.2019. Optimizing methods for human testicular tissue cryopreservation and spermatogonial stem cell isolation. Journal of Cellular Biochemistry, 120 (1): 613-621. |
| null | Morrell J M, Hodges J K. 1998. Cryopreservation of non?human primate sperm: priorities for future research. Animal Reproduction Science, 53 (1-4): 43-63. |
| null | Motohashi H H, Ishibashi H. 2016. Cryopreservation of ovaries from neonatal marmoset monkeys. Experimental Animals, 65 (3): 189-196. |
| null | Moussa M, Shu J, Zhang X H, Zeng F Y. 2014. Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives. Science China Life Sciences, 57 (9): 903-914. |
| null | Mukunoki A, Takeo T, Nakao S, Tamura K, Horikoshi Y, Nakagata N. 2020. Simple transport and cryopreservation of cold?stored mouse embryos. Experimental Animals, 69 (4): 423-429. |
| null | Nichols S M, Bavister B D. 2006. Comparison of protocols for cryopreservation of rhesus monkey spermatozoa by post-thaw motility recovery and hyperactivation. Reproduction, Fertility and Development, 18 (7): 777-780. |
| null | Oliveira K G, Le?o D L, Almeida D V, Santos R R, Domingues S F. 2015. Seminal characteristics and cryopreservation of sperm from the squirrel monkey, Saimiri collinsi . Theriogenology, 84 (5): 743-749. |
| null | Oliveira K G, Miranda S A, Le?o D L, Brito A B, Santos R R, Domingues S F. 2011. Semen coagulum liquefaction, sperm activation and cryopreservation of capuchin monkey (Cebus apella) semen in coconut water solution (CWS) and TES-TRIS. Animal Reproduction Science, 123 (1-2): 75-80. |
| null | Oliveira K G, Santos R R, Le?o D L, Brito AB, Lima J S, Sampaio W V, Domingues S F. 2016. Cooling and freezing of sperm from captive, free?living and endangered squirrel monkey species. Cryobiology, 72 (3): 283-289. |
| null | Pampanini V, Hassan J, Oliver E, Stukenborg J B, Damdimopoulou P, Jahnukainen K. 2020.Fertility preservation for prepubertal patients at risk of infertility: present status and future perspectives. Hormone Research in Paediatrics, 93 (11-12): 599-608. |
| null | Pegg D E. 2015. Principles of cryopreservation. Methods in Molecular Biology, 1257: 3-19. |
| null | Poels J, Van Langendonckt A, Dehoux J P, Donnez J, Wyns C. 2012. Vitrification of non?human primate immature testicular tissue allows maintenance of proliferating spermatogonial cells after xenografting to recipient mice. Theriogenology, 77 (5): 1008-1013. |
| null | Pope C E, Pope V Z, Beck L R. 1984. Live birth following cryopreservation and transfer of a baboon embryo. Fertility and Sterility, 42 (1): 143-145. |
| null | Pope C E, Pope V Z, Beck L R. 1986. Cryopreservation and transfer of baboon embryos. In Vitro Fertilization and Embryo Transfer, 3 (1): 33-39. |
| null | Pothana L, Venna N K, Devi L, Singh A, Chatterjee I, Goel S. 2016. Cryopreservation of adult primate testes. European Journal of Wildlife Research, 62: 619-626. |
| null | Rylands A B, Mittermeier R A. 2014. Primate taxonomy: species and conservation. Evolutionary Anthropology, 23 (1): 8-10. |
| null | Sánchez?Partida L G, Maginnis G, Dominko T, Martinovich C, McVay B, Fanton J, Schatten G. 2000. Live rhesus offspring by artificial insemination using fresh sperm and cryopreserved sperm. Biology of Reproduction, 63 (4): 1092-1097. |
| null | Schneider F, Redmann K, Wistuba J, Schlatt S, Kliesch S, Neuhaus N.2015. Comparison of enzymatic digestion and mechanical dissociation of human testicular tissues. Fertility and Sterility, 104 (2): 302-311. |
| null | Seier J V, Conradie E, Oettle E E, Fincham J E. 1993. Cryopreservation of vervet monkey semen and recovery of progressively motile spermatozoa. Journal of Medical Primatology, 22 (6): 355-359. |
| null | Shetty G, Mitchell J M, Lam T N A, Wu Z, Zhang J, Hill L, Tailor R C, Peters K A, Penedo M C, Orwig K E, Meistrich M L. 2018. Donor spermatogenesis in de novo formed seminiferous tubules from transplanted testicular cells in rhesus monkey testis. Human Reproduction, 33 (12): 2249-2255. |
| null | Shetty G, Mitchell J M, Meyer J M, Wu Z, Lam T N A, Phan T T, Zhang J, Hill L, Tailor R C, Peters K A, Penedo M C, Hanna C, Orwig K E, Meistrich M L. 2020. Restoration of functional sperm production in irradiated pubertal rhesus monkeys by spermatogonial stem cell transplantation. Andrology, 8 (5): 1428-1441. |
| null | Shi X W, Hu H H, Ji G J, Zhang J, Liu R, Zhang H, Li M W. 2018. Protective effect of sucrose and antioxidants on cryopreservation of sperm motility and DNA integrity in C57BL/6 mice. Biopreservation and Biobanking, 16 (6): 444-450. |
| null | Si W, Zheng P, Li Y H, Dinnyes A, Ji W Z. 2004. Effect of glycerol and dimethyl sulfoxide on cryopreservation of rhesus monkey (Macaca mulatta) sperm. American Journal of Primatology, 62 (4): 301-306. |
| null | Silva A M D, Pereira A F, Comizzoli P, Silva A R. 2020. Cryopreservation and culture of testicular tissues: an essential tool for biodiversity preservation. Biopreservation and Biobanking, 18 (3): 235-243. |
| null | Strelchenko N S, Schmidt J K, Mean K D, Schotzko M L, Golos T G, Slukvin I I. 2020. Cryopreservation of mauritian cynomolgus macaque (Macaca fascicularis) sperm in chemically defined medium. Journal of the American Association for Laboratory Animal Science, 59 (6): 681-686. |
| null | Summers P M, Shephard A M, Taylor C T, Hearn J P. 1987. The effects of cryopreservation and transfer on embryonic development in the common marmoset monkey, Callithrix jacchus . Journal of Reproduction and Fertility, 79 (1): 241-250. |
| null | Suzuki N, Hashimoto S, Igarashi S, Takae S, Yamanaka M, Yamochi T, Takenoshita M, Hosoi Y, Morimoto Y, Ishizuka B. 2012. Assessment of long?term function of heterotopic transplants of vitrified ovarian tissue in cynomolgus monkeys. Human Reproduction, 27 (8): 2420-2429. |
| null | Takasu M, Morita N, Tajima S, Almunia J, Maeda M, Kamiguchi T. 2016. Cryopreservation of lar gibbon semen collected by manual stimulation. Primates, 57 (3): 303-307. |
| null | Thomson L K, Fleming S D, Aitken R J, de Luliis G N, Zieschang J A, Clark A M. 2009. Cryopreservation?induced human sperm DNA damage is predominantly mediated by oxidative stress rather than apoptosis. Human Reproduction, 24 (9): 2061-2070. |
| null | Ting A Y, Yeoman R R, Campos J R, Lawson M S, Mullen S F, Fahy G M, Zelinski M B. 2013. Morphological and functional preservation of pre?antral follicles after vitrification of macaque ovarian tissue in a closed system. Human Reproduction, 28 (5): 1267-1279. |
| null | Tollner T L, Dong Q, VandeVoort C A. 2011. Frozen?thawed rhesus sperm retain normal morphology and highly progressive motility but exhibit sharply reduced efficiency in penetrating cervical mucus and hyaluronic acid gel. Cryobiology, 62 (1): 15-21. |
| null | Tollner T L, VandeVoort C A, Overstreet J W, Drobnis E Z. 1990. Cryopreservation of spermatozoa from cynomolgus monkeys (Macaca fascicularis). Journal of Reproduction and Fertility, 90 (2): 347-352. |
| null | Tomioka I, Takahashi T, Shimada A, Yoshioka K, Sasaki E. 2012. Birth of common marmoset (Callithrix jacchus) offspring derived from in vitro-matured oocytes in chemically defined medium. Theriogenology, 78 (7): 1487-1493. |
| null | Picton H M.2018.Preservation of female fertility in humans and animal species. Animal Reproduction, 15 (3): 301-309. |
| null | VandeVoort C A, Leibo S.2005. Effect of cooling and exposure to ethylene glycol on in vitro maturation and embryo development of rhesus oocytes. Cryo Letters, 26: 305-312. |
| null | VandeVoort C A, Shirley C R, Hill D L, Leibo S. 2008. Effects of cryoprotectants and cryopreservation on germinal vesicle-stage cumulus?oocyte complexes of rhesus monkeys. Fertility and Sterility, 90: 805-816. |
| null | Wagner M, Yoshihara M, Douagi I, Damdimopoulos A, Panula S, Petropoulos S, Lu H, Pettersson K, Palm K, Katayama S, Hovatta O, Kere J, Lanner F, Damdimopoulou P. 2020. Single?cell analysis of human ovarian cortex identifies distinct cell populations but no oogonial stem cells. Nature Communications, 11 (1): 1147. |
| null | White Y A, Woods D C, Takai Y, Ishihara O, Seki H, Tilly J L. 2012. Oocyte formation by mitotically active germ cells purified from ovaries of reproductive?age women. Nature Medicine, 18 (3): 413-421. |
| null | Yamasaki J, Iwatani C, Tsuchiya H, Okahara J, Sankai T, Torii R. 2011. Vitrification and transfer of cynomolgus monkey (Macaca fascicularis) embryos fertilized by intracytoplasmic sperm injection. Theriogenology, 76 (1): 33-38. |
| null | Yeoman R R, Gerami?Naini B, Mitalipov S, Nusser K D, Widmann‐Browning A A, Wolf D P. 2001. Cryoloop vitrification yields superior survival of rhesus monkey blastocysts. Human Reproduction, 16 (9): 1965-1969. |
| null | Yu J F, Lai Y H, Wang T E, Wei Y S, Chang Y J, Li S H, Chin S C, Joshi R, Chang H W, Tsai P S. 2018. The effects of typeⅠcollagenase on the degelification of chimpanzee (Pan troglodytes) semen plug and sperm quality. BMC Veterinary Research, 14 (1): 58. |
| null | Zini A, Boman J M, Belzile E, Ciampi A. 2008. Sperm DNA damage is associated with an increased risk of pregnancy loss after IVF and ICSI: systematic review and meta?analysis. Human Reproduction, 23 (12): 2663-2668. |
| null | Zinner D, Roos C. 2016. Primate taxonomy and conservation. In: Waller M ed. Ethnoprimatology. Developments in Primatology: Progress and Prospects. Springer, Cham. |
| null | 尹峰, 梦梦, 刘定震, 周岐海, 黄乘明. 2015. 我国非人灵长类野外生态学研究现状与展望. 林业资源管理, 4: 3-8, 14. |
| null | 王宏, 付学魏, 陈智岗, 宗发梁, 李鹤龄, 韦秋奖. 2017. 昆明地区恒河猴、食蟹猴种群繁殖规律和繁殖性能研究.中国比较医学杂志, 7: 34-39. |
| null | 石晓卫, 胡焕焕, 姬国杰, 李明文. 2019. 卵低温保存过程中透明带提早硬化的影响因素和调控机制.中华生殖与避孕杂志, 39 (2): 156-160. |
| null | 采克俊, 李亚辉, 李剑, 和协超, 季维智. 2005.不同渗透压的稀释液对猕猴精子低温冷冻保存的影响.动物学研究, 26 (3): 305-310. |
| null | 黄璋琼, 薛志刚, 高家红, 江勤芳, 吴正存, 李聪, 罕园园, 曾桥, 马开利.2014. 食蟹猴和恒河猴卵母细胞及早期胚胎玻璃化冻存.中国细胞生物学学报, 36 (7): 949-955. |
| null | 中国野生动物保护协会. 中国非人灵长类的现状与保护. 2020-11-16. |
| null | 马世来, 王应祥.1988.中国现代灵长类的分布、现状和保护. 兽类学报, 8 (4): 250-260. |
| null | 范朋飞. 2012.中国长臂猿科动物的分类和保护现状.兽类学报, 32 (3): 248-258. |
| null | 张荣祖, 全国强, 赵体恭, Southwick C H.1992.灵长类 (除猕猴属外) 在中国的分布.兽类学报, 12 (2): 3-17. |
/
| 〈 |
|
〉 |
青公网安备 63010402000199号 青ICP备05000010号-2
