高海拔环境抑制高原鼢鼠和高原鼠兔的胆汁酸代谢

张佳钰; 安志芳; 王志洁; 陈晓琦; 魏登邦

doi:10.16829/j.slxb.150805

兽类学报 >

2023 , Vol. 43 >Issue 5: 553 - 567

DOI: https://doi.org/10.16829/j.slxb.150805

研究论文

高海拔环境抑制高原鼢鼠和高原鼠兔的胆汁酸代谢

张佳钰 ,
安志芳 ,
王志洁 ,
陈晓琦 ,
魏登邦

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1 青海大学生态环境工程学院, 西宁 810016;
2 青海大学省部共建三江源生态与高原农牧业国家重点实验室, 西宁 810016

张佳钰(1996-)，女，硕士研究生，主要从事动物生态学研究.

收稿日期: 2023-04-25

修回日期: 2023-06-20

网络出版日期: 2023-09-22

基金资助

第二次青藏高原综合科学考察研究项目(2019QZKK0501)；青海省自然科学基金(2021-ZJ-904)

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Inhibition of bile acid metabolism in plateau zokor (Myospalax baileyi) and plateau pika (Ochotona curzoniae) in high-altitude environments

ZHANG Jiayu ,
AN Zhifang ,
WANG Zhijie ,
CHEN Xiaoqi ,
WEI Dengbang

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1 College of Eco-Environmental Engineering, Qinghai University, Xining 810016, China;
2 State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, China

Received date: 2023-04-25

Revised date: 2023-06-20

Online published: 2023-09-22

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摘要

高原鼢鼠和高原鼠兔是青藏高原特有动物，低氧寒冷的环境影响其生理活动。胆汁酸是一种具有两亲特性的固醇类衍生物，不仅有利于脂溶性营养成分和维生素吸收，而且作为信号激素参与组织器官的糖脂代谢和能量代谢，具有调节功能，与动物适应环境有关。本文应用靶向代谢组学和荧光PCR定量方法，以Sprague-Dawley (SD)大鼠为对照，比较分析了SD大鼠、高原鼢鼠(Myospalax baileyi)和高原鼠兔(Ochotona curzoniae)血清中胆汁酸的组成和含量以及肝脏中初级胆汁酸合成关键酶基因的表达水平。结果表明，高原鼢鼠和高原鼠兔血清总胆汁酸含量均显著低于SD大鼠；随海拔升高，两种高原动物血清胆汁酸含量均显著下降，高原鼢鼠显著低于高原鼠兔，肝脏中初级胆汁酸合成关键酶基因CYP7A1、CYP8B1、CYP27A1和CYP7B1表达水平均显著下降；SD大鼠初级胆汁酸主要通过经典途径合成，而高原鼢鼠和高原鼠兔初级胆汁酸主要通过替代途径合成，提示高海拔环境抑制胆汁酸合成，低氧可能是主要的抑制因子。两种高原动物血清胆汁酸以非12-羟基胆汁酸和非结合型次级胆汁酸为主，结合型胆汁酸以甘氨酸结合型为主。特别是在高原鼢鼠血清中，石胆酸(lithocholic acid,LCA)类胆汁酸含量显著高于高原鼠兔和SD大鼠，这可能与其适应高耗能的地下挖掘生活有关。总之，高原鼢鼠和高原鼠兔初级胆汁酸合成受低氧抑制且以替代途径为主，这可能是它们长期适应高原环境的结果。

关键词： 高原鼢鼠; 高原鼠兔; 血清; 胆汁酸; 低氧; 胆汁酸合成关键酶基因

本文引用格式

张佳钰 , 安志芳 , 王志洁 , 陈晓琦 , 魏登邦 . 高海拔环境抑制高原鼢鼠和高原鼠兔的胆汁酸代谢[J]. 兽类学报, 2023 , 43(5) : 553 -567 . DOI: 10.16829/j.slxb.150805

Abstract

The plateau zokor (Myospalax baileyi) and plateau pika (Ochotona curzoniae), which are native to the Qinghai-Xizang Plateau, inhabit hypoxic meadows that pose unique physiological challenges. Bile acids are amphipathic steroid metabolites that not only facilitate intestinal absorption of lipids and fat-soluble vitamins, but also act as hormones capable of reaching virtually every organ to regulate glycometabolism, lipid metabolism, and energy homeostasis, which are related to the adaptation of animals to the environments. In this study, the composition and contents of bile acids in the serum of plateau zokors and plateau pikas were analyzed by targeting metabolomics. The Sprague-Dawley (SD) rats were used as control and the expression levels of the key genes of bile acids anabolism were determined by real-time PCR. The results showed that the total bile acid contents in the serum of plateau zokors and plateau pikas decreased markedly than that of SD rats. With increasing altitude, the bile acid contents of zokors and pikas decreased significantly, and that of zokors reduced remarkably compared to that of pikas. The expression levels of the key genes of primary bile acids including CYP7A1, CYP8B1, CYP27A1, and CYP7B1 decreased significantly in livers of zokors and pikas. The bile acids of SD rats were synthesized by the classic or neutral bile acid pathway, but that of zokors and pikas were produced by the alternative or acidic pathway. These results suggest that high-altitude environments inhibit the anabolism of primary bile acids and that hypoxia might be the main inhibiting factor. The no-12-OH bile acids and the unconjugated secondary bile acids were the main products and the glycine conjugated bile acids were the main bile acids in the serum of zokors and pikas. Particularly, the contents of lithocholic acid (LCA) bile acids were significantly higher than that of pikas and SD rats in serum which might be related to its adaptation to the higher energy expenditure of burrowing activities. In conclusion, the alternative pathway was the dominant pathway of primary bile acids in the liver of zokors and pikas, which might be a result of long-time adaptation to the highland environments.

Key words： Plateau zokor (Myospalax baileyi); Plateau pika (Ochotona curzoniae); Serum; Bile acids; Hypoxia; Key enzyme genes for bile acid synthesis

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