同一笼养生境下金头狮面狨猴、赤掌柽柳猴和棉顶狨猴肠道菌群比较

张泽明; 姚红玉; 吴楠; 赵玲玲; 顾逸如; 王宇; 吴弘; 赵大鹏

doi:10.16829/j.slxb.150739

兽类学报 >

2023 , Vol. 43 >Issue 5: 580 - 592

DOI: https://doi.org/10.16829/j.slxb.150739

研究论文

同一笼养生境下金头狮面狨猴、赤掌柽柳猴和棉顶狨猴肠道菌群比较

张泽明 ,
姚红玉 ,
吴楠 ,
赵玲玲 ,
顾逸如 ,
王宇 ,
吴弘 ,
赵大鹏

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1 天津师范大学生命科学学院, 天津 300387;
2 南京市红山森林动物园, 南京 210028

张泽明(1997-)，男，硕士研究生，主要从事野生动物资源保护研究.

收稿日期: 2022-10-10

修回日期: 2023-05-16

网络出版日期: 2023-09-22

基金资助

国家自然科学基金(31772468)；中央财政林业补助资金项目-野生动物监测专项；天津市规划和自然资源局项目

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Comparison of intestinal microbiota of golden-headed lion tamarins, golden-handed tamarins and cotton-headed tamarins under the same captive environment

ZHANG Zeming ,
YAO Hongyu ,
WU Nan ,
ZHAO Lingling ,
GU Yiru ,
WANG Yu ,
WU Hong ,
ZHAO Dapeng

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1 College of Life Sciences, Tianjin Normal University, Tianjin 300387, China;
2 Nanjing Hongshan Forest Zoo, Nanjing 210028, China

Received date: 2022-10-10

Revised date: 2023-05-16

Online published: 2023-09-22

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摘要

动物的肠道菌群组成与宿主种类及其生存环境直接相关，研究同一生境下不同物种的肠道菌群组成对于物种多样性保护具有积极意义。本研究采用非损伤取样和16S rDNA高通量测序等方法，以南京市红山森林动物园笼养的金头狮面狨猴(Leontopithecus chrysomelas)、赤掌柽柳猴(Saguinus midas)和棉顶狨猴(Saguinus oedipus)三种新大陆猴为研究对象，比较分析其肠道菌群结构组成差异，并进行功能预测。结果发现：3个物种的肠道菌群合计14门20纲50目101科231属356种。优势菌门均为厚壁菌门(Firmicutes)、放线菌门(Actinobacteria)、变形菌门(Proteobacteria)和拟杆菌门(Bacteroidetes)，共有优势菌属为链球菌属(Streptococcus)和双歧杆菌属(Bifidobacterium)。3个物种间肠道菌群在门水平和属水平均存在显著差异，其中金头狮面狨猴的肠道菌群丰富度显著高于另外两个物种。本研究结果为灵长类肠道菌群多样性与生存适应研究提供基础数据，同时为相关物种的易地保护管理提供参考依据。

关键词： 新大陆猴; 肠道菌群; 高通量测序技术; 16S rDNA

本文引用格式

张泽明 , 姚红玉 , 吴楠 , 赵玲玲 , 顾逸如 , 王宇 , 吴弘 , 赵大鹏 . 同一笼养生境下金头狮面狨猴、赤掌柽柳猴和棉顶狨猴肠道菌群比较[J]. 兽类学报, 2023 , 43(5) : 580 -592 . DOI: 10.16829/j.slxb.150739

Abstract

The composition of intestinal microbiota in animals is directly related to the host species and their living environment. Studies on intestinal microbiota composition of various species living in the same habitat is important for the conservation of species diversity. Both non-damage sampling and 16S rDNA high-throughput sequencing were used to investigate the structure, diversity, and functional prediction of intestinal microbiota across three species of New World monkeys (golden-headed lion tamarin Leontopithecus chrysomelas, golden-handed tamarin Saguinus midas, and cottonheaded tamarin Saguinus oedipus) from Nanjing Hongshan Forest Zoo. The results showed that a total of 14 phyla, 20 classes, 50 orders, 101 families, 231 genera, and 356 species of intestinal flora were obtained from 18 fecal samples collected from the three species. At the phylum level, the common dominant phyla across the three species were Firmicutes, Actinobacteria, Proteobacteria, and Bacteroidetes. At the genus level, the common dominant bacterial genera included Streptococcus and Bifidobacterium. There were significant differences in intestinal microbiota at both the phylum level and the genus level among species, in which the richness of intestinal microbiota in golden-headed lion tamarin was significantly higher than that in the others. This study provides basic data for further investigation on the intestinal microbiota diversity and survival adaptation, and also serves as reference for ex situ management for species conservation.

Key words： New world monkeys; Intestinal microbiota; High-throughput sequencing; 16S rDNA

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