藏羚交配群成员间亲缘关系及遗传多样性分析

赛青高娃; 王子涵; 李全邦; 王东; 连新明

doi:10.16829/j.slxb.150807

兽类学报 >

2024 , Vol. 44 >Issue 2: 159 - 170

DOI: https://doi.org/10.16829/j.slxb.150807

研究论文

藏羚交配群成员间亲缘关系及遗传多样性分析

赛青高娃 ,
王子涵 ,
李全邦 ,
王东 ,
连新明

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1 中国科学院西北高原生物研究所, 高原生物适应与进化重点实验室, 西宁 810001;
2 中国科学院大学, 北京 100049;
3 青海师范大学地理科学学院, 西宁 810016;
4 青海省动物生态基因组学重点实验室, 西宁 810001

赛青高娃(1998-)，女，硕士，主要从事保护生物学研究.

收稿日期: 2023-05-02

修回日期: 2024-01-31

网络出版日期: 2024-04-03

基金资助

国家重点研发计划项目(2023YFF1305000)；青海省自然科学与工程技术学科带头人专项资金；中国科学院“西部之光”青年学者A类项目

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Parentage testing and genetic diversity of Tibetan antelope individuals in or among mating groups

Saiqinggaowa ,
WANG Zihan ,
LI Quanbang ,
WANG Dong ,
LIAN Xinming

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1 Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China;
2 University of Chinese Academy of Sciences, Beijing 100049, China;
3 College of Geographical Sciences, Qinghai Normal University, Xining 810016, China;
4 Qinghai Provincial Key Laboratory of Animal Ecological Genomics, Xining 810001, China

Received date: 2023-05-02

Revised date: 2024-01-31

Online published: 2024-04-03

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摘要

藏羚 (Pantholops hodgsonii) 是青藏高原特有物种，多集群生活且具有典型的性别分离现象。除交配季节外，雌雄两性个体组成的同性集群分开活动。本研究于 2021 年 12 月下旬在三江源国家公园可可西里片区以藏羚集群为单元采集了 32 个集群共 188 份新鲜粪便样品，利用多态性较高的 10 个微卫星位点进行亲缘关系鉴定与遗传多样性分析。结果表明：(1) 188 份新鲜粪便样品来自 145 只藏羚个体，其中 10 只藏羚个体 (8 只雌羚，2 只雄羚) 出现更换集群现象，导致前后集群发生变化。结合野外实地记录，推测藏羚交配群的变化存在 3 种方式：集群解散，雄性个体离开 (加入)，雌性个体离开 (加入)。新加入的藏羚个体与原集群成员间的亲缘关系较远。雄性藏羚更换的集群中雌性个体均多于之前的集群，能够获得更多交配机会。(2) 10 个微卫星位点的平均等位基因数为 16. 1，平均多态信息含量为 0. 766。观测杂合度(Ho) 0. 607 ~ 0. 993，平均值为 0. 819；期望杂合度 (He) 0. 575 ~0. 930，平均值为 0. 798，表明藏羚种群遗传多样性丰富。(3) 经过亲缘关系鉴定，种群内所有亲子关系中 14 对(43. 75%) 发生在集群内并且以母女 (71. 43%)为主，与雄性个体相关的亲子对 (母子/父女/父子) 有 4 对 (28. 57%)。对比集群内及集群间藏羚的平均亲缘系数，结果表明雄性个体对集群内成员间的亲缘关系影响不大。针对集群间藏羚亲缘关系的研究结果也表明，藏羚种群的近交比例处于较低水平。由于藏羚雌性比雄性个体有更多时间和机会组成含有多个雌性成员的集群，因此，集群内雌性个体的亲缘关系较高不仅有利于提高集群的稳定性，还有利于迁徙信息的交流和传递，从而为进一步验证藏羚迁徙的集体记忆猜想提供科学佐证。

关键词： 藏羚; 粪便DNA; 交配群; 亲缘关系; 遗传多样性

本文引用格式

赛青高娃 , 王子涵 , 李全邦 , 王东 , 连新明 . 藏羚交配群成员间亲缘关系及遗传多样性分析[J]. 兽类学报, 2024 , 44(2) : 159 -170 . DOI: 10.16829/j.slxb.150807

Abstract

Tibetan antelope (Pantholops hodgsonii), an endemic species of the Qinghai-Tibet Plateau, exhibits group living and sexual segregation behaviors. Outside the rutting season, male and female Tibetan antelope live separately. In this study, a total of 188 fresh fecal samples were collected from 32 groups of Tibetan antelope in the Kekexili region of the Sanjiangyuan National Park in late December 2021. Genetic diversity analysis and parentage testing were conducted using ten highly polymorphic microsatellite loci. The findings revealed that out of the 188 fresh fecal samples, genetic identification confirmed the presence of 145 individual Tibetan antelope. Among them, ten Tibetan antelope individuals (eight females and two males) sequentially appeared in different groups over time. Field observations indicated three mechanisms for recombination among mating groups including group dissolution, male departures or arrivals to/from existing groups, and female departures or arrivals to/from existing groups. Notably, there was considerable genetic differentiation between individuals that changed groups compared to those remaining within their original group composition. Male Tibetan antelopes moved to groups with a higher proportion of females than their previous ones, thereby increasing their potential for acquiring more mates and enhancing opportunities for gene inheritance. The average number of alleles across the ten microsatellite loci was 16. 1, with an average polymorphic information content of 0. 766. Observed heterozygosity (Ho) ranged from 0. 607 to 0. 993 with a mean value of 0. 819. Expected heterozygosity (He) ranged from 0. 575 to 0. 930 with a mean value of 0. 798. These results indicated a high level of genetic diversity within the Tibetan antelope population. At the group level, out of the total parent-child relationships observed (n = 32), fourteen (43. 75%) occurred within the same group, predominantly in mother-daughter relationships (71. 43 %), while only four (28. 57%) involved males (mother-son/father-daughter/father-son). By comparing the average coefficients of relatedness between groups with or without males, our findings suggested that the presence or absence of males had minimal impact on genetic relatedness among group members, indicating that closest genetic relationships within mating groups were primarily among females. Our findings demonstrated that low levels of inbreeding in Tibetan antelope populations and highlight female-female associations as key drivers for forming stable groups and facilitating communication and transmission of migration information due to their higher relatedness compared to males. These results provided scientific evidence supporting further validation of the collective memory conjecture regarding Tibetan antelope migration.

Key words： Tibetan antelope; Fecal DNA; Mating group; Parentage testing; Genetic diversity

参考文献

Bellemain E, Swenson J E, Tallmon D A, Brunberg S, Taberlet P. 2005. Estimating population size of elusive animals with DNA from hunter-collected feces:Four methods for Brown Bears.Conservation Biology, 19 (1):150-161.
Bishop M D, Kappes S M, Keele J W, Stone R T, Sunden S L F, Hawkins G A, Toldo S S, Fries R, Grosz M D, Yoo J, Beattie C W. 1994. A genetic linkage map for cattle. Genetics, 136 (2):619-639.
Bon R, Campan R. 1996. Unexplained sexual segregation in polyga mous ungulates:a defense of an ontogenetic approach. Behav ioural Processes, 38 (2):131-154.
Botstein D, White R, Skolnick M H, Davis R W. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics, 32 (3):314-331.
Brezinsky L, Kemp S J, Teale A J. 1993. Five polymorphic bovine mi crosatellites (ILSTS010-014). Animal Genetics, 24 (1):75-76.
Chen X N. 2014. The mating system and home range of black muntjac based on fecal DNA. Master thesis. Jinhua:Zhejiang Normal University. (in Chinese)
Clutton-Brock T. 2009. Structure and function in mammalian societies. Philos Trans R Soc Lond B Biol Sci, 364 (1533):3229-3242.
Gong L, Wang F K, Li J F, Ye Y J, Li L H, Deng S Y. 2020. Character istics of soil wind erosion and analysis of meteorological influencing factors in Wudaoliang area. Qinghai Prataculture, 29 (3):59-63. (in Chinese)
Guo K. 1993. Vegetation of Qinghai Hoh Xil region. Acta Phytoecologica et Geobotanica Sinica, (2):26-38. (in Chinese)
Hamilton W D. 1963. The evolution of altruistic behavior. The American Naturalist, 97 (896):354-356.
Hamilton W D. 1964. The genetical evolution of social behaviour. II.Journal of Theoretical Biology, 7 (1):17-52.
Hong Y Y, Li D Q, Yi T Y, Zhang Y G, Liu Y. 2018. Screening micro satellite primers and their application for individual identification in feces DNA from Procapra praprzewalskii. Chinese Agricultural Science Bulletin, 24 (9):54-58. (in Chinese)
Hu J. 2001. Study on the biological characteristics and genetic diversities of wild yak and domestic yak and their hybrid. Master the sis. Lanzhou:Gansu Agricultural University. (in Chinese)
Inoue M, Takenaka O. 2006. Japanese macaque microsatellite PCR primers for paternity testing. Primates, 34 (1):37-45.
Jin Y C. 2012. The genetic structure and home range of wapiti based on fecal molecular biology. Master thesis. Harbin:Northeast For estry University. (in Chinese)
Kalinowski S T, Taper M L, Marshall T C. 2007. Revising how the computer program CERVUS accommodates genotyping error in creases success in paternity assignment. Molecular Ecology, 16(5):1099-1106.
Konovalov D A, Manning C, Henshaw M T. 2004. KINGROUP:a program for pedigree relationship reconstruction and kin group assignments using genetic markers. Molecular Ecology Notes, 4(4):779-782.
Lian X M, Su J P, Zhang T Z, Cao Y F. 2005. The characteristics of social groups of the Tibetan antelope (Pantholops hodgsonii) in the Kekexili region. Acta Ecologica Sinica, 25 (6):1341-1346.(in Chinese)
Li K, Geng J, Qu J, Zhang Y, Hu S. 2010. Effectiveness of 10 polymorphic microsatellite markers for parentage and pedigree analysis in plateau pika (Ochotona curzoniae). BMC Genetics, 11:101.
Lin T T. 2017. Isolation of microsatellite markers and population genetic diverdity analysis in Mastacembelus armatus. Master the sis. Guangzhou:Guangzhou University. (in Chinese)
Ma S J. 2007. Current situation investigation of grassland resource in Tanggula Mountain and Kekexili in Qinghai-Tibet Plateau.Pratacultural Science, 24 (9):15-19. (in Chinese)
Miao Z Y, Wu T, Chen J P, Wang D, Li H Q, Lian X M. 2020. Effect of the Qinghai-Tibet Railway on diurnal behavioural time bud gets in male Tibetan antelopes. Acta Theriologica Sinica, 40 (2):135-142. (in Chinese)
Myers E M, Zamudio K R. 2004. Multiple paternity in an aggregate breeding amphibian:the effect of reproductive skew on estimates of male reproductive success. Molecular Ecology, 13:1951-1963.
Nesje M, Røed K H, Lifjeld J T, Lindberg P, Steen O F. 2000. Genetic relationships in the peregrine falcon (Falco peregrinus) analyzed by microsatellite DNA markers. Molecular Ecology, 9 (1):53-60.
Qu K X, Li H W, He Z X, Yuan X P, Huang B Z. 2008. Microsatellite DNA marker and paternity testing. China Cattle Science, 34 (6):43-46. (in Chinese)
Rao K B C A, Rao V K, Kowale B N, a S M T. 1995. Sex-specific identification of raw meat from cattle, buffalo, sheep and goat.Meat Science, 39 (1):123-126.
Ren P. 2016. The parentage test, mating system and population quantity of muntiacus reevesi based on fecal DNA. Master thesis. Jin hua:Zhejiang Normal University. (in Chinese)
Ren Z J, Xiong J J, Deng Z J, He T, Song X X, Jin C, Li J L, Wang Y, Zhang H. 2011. Genetic polymorphism analysis by microsatellite markers in white-lipped deer. Acta Agriculturae Borealioccidentalis Sinica, 20 (5):6-9. (in Chinese)
Ruckstuhl K E. 1999. To synchronise or not to synchronise:a di lemma for young bighorn males? Behaviour, 136 (6):805-818.
Saiqinggaowa, Li H Q, Wang D, Wang Z H, Lian X M. 2022. The utilization of wildlife passages by migratory Tibetan antelope in San jiangyuan National Park:a case study of Wubei Bridge of Qinghai Tibet Railway. Acta Theriologica Sinica, 42 (5):601-608. (in Chinese)
Schaller G B. 1998. Wildlife of the Tibetan Steppe. Chicago:the University of Chicago Press.
Schaller G B, Kang A L, Cai X B, Liu Y L. 2006. Migratory and calving behavior of Tibetan antelope population. Acta Theriologica Sinica, 26 (2):105-113.
Su J P, Chen J R, Lian X M, Zhang T Z. 2017. Speculation on the causes of seasonal migration of Tibetan antelope. World Heritages, 8:79-85. (in Chinese)
Webster M S, Westneat D F. 1998. The use of molecular markers to study kinship in birds:techniques and questions. In:DeSalle R, Schierwater B eds. Molecular Approaches to Ecology and Evolution. Basel:Springer Basel AG.
Weikard R, Pitra C, Kuhn C. 2006. Amelogenin cross amplification in the family Bovidae and its application for sex determination. Mo lecular Reproduction and Development, 73 (10):1333-1337.
Wu J F. 2002. Application of microsatellite DNA in parentage testing of Liangshan semi-fine wool sheep. Master thesis. Ya'an:Sichuan Agricultural University. (in Chinese)
Wu T. 2021. Sexual segregation and male mating tactics in Tibetan an telope. Ph. D thesis. Xining:Northwest Institute of Plateau Biol ogy, Chinese Academy of Sciences. (in Chinese)
Wu T, Lian X M, Miao Z Y, Li H Q, Chen J P, Wang D, Zhang T Z. 2021. Ethogram and PAE coding system for Tibetan antelope(Pantholops hodgsonii). Chinese Journal of Ecology, 40 (5):1449-1457. (in Chinese)
Wu Y H. 2007. Adaptation of migration of female Tibetan antelope for precipitation distribution on the Qinghai-Tibet Plateau. Acta Theriologica Sinica, 27 (3):298-307. (in Chinese)
Zane L, Bargelloni L, Patarnello T. 2002. Strategies for microsatellite isolation:a review. Molecular Ecology, 11:1-16.
Zhou H, Li D, Zhang Y, Yang T, Liu Y. 2007. Genetic diversity of mi crosatellite DNA loci of Tibetan antelope (Chiru, Pantholops hodgsonii) in Hoh Xil National Nature Reserve, Qinghai, China.Journal of Genetics and Genomics, 34 (7):600-607.
Zhou Z H. 2014. Trends in variation of genetic diversity in Tibetan an telope (Pantholops hodgsonii) during the past decade based on microsatellite markers. Master thesis. Xining:Qinghai Normal University. (in Chinese)
Zhou Z H, Guo S Z, Ma J B, Xu Y T, Tan C M, Du Y R. 2014. Genetic diversity of endangered Tibetan antelope revealed by micro satellite markers. Journal of Qinghai Normal University (Natural Science), 30 (2):36-39. (in Chinese)
亐开兴, 李红伟, 和占星, 袁希平, 黄必志. 2008. 微卫星 DNA 标记与亲子鉴定. 中国牛业科学, 34 (6):43-46.
马松江. 2007. 青藏高原唐古拉山及可可西里草地资源现状调查研究. 草业科学, 24 (9):15-19.
巩俐, 王发科, 李积芳, 冶云娟, 李路华, 邓生寅. 2020. 五道梁地区土壤风蚀变化特征及气象影响因子分析. 青海草业, 29 (3):59-63.
任战军, 熊建杰, 邓铸疆, 何婷, 宋旭旭, 靳程, 李军亮, 王禹, 张慧. 2011. 白唇鹿的微卫星遗传多态性分析. 西北农业学报, 20(5):6-9.
任鹏. 2016. 基于粪便 DNA 的小麂亲权鉴定、婚配制及种群数量研究. 金华:浙江师范大学硕士学位论文.
陈小南. 2014. 基于粪便 DNA 的黑麂婚配制及家域的研究. 金华:浙江师范大学硕士学位论文.
吴彤. 2021. 藏羚性别分离机制及雄性交配对策研究. 西宁:中国科学院大学博士学位论文.
吴彤, 连新明, 苗紫燕, 李宏奇, 陈佳萍, 王东, 张同作. 2021. 藏羚行为的 PAE 编码系统. 生态学杂志, 40 (5):1449-1457.
苏建平, 陈家瑞, 连新明, 张同作. 2017. 藏羚羊季节性迁徙成因猜想. 世界遗产, 8:79-85.
吴继法. 2002. 微卫星 DNA 在凉山半细毛羊亲子鉴定中的应用.
雅安:四川农业大学硕士学位论文.
连新明, 苏建平, 张同作, 曹伊凡. 2005. 可可西里地区藏羚的社群特征. 生态学报, 25 (6):1341-1346.
武永华. 2007. 雌性藏羚迁徙对青藏高原降水时空分布的适应性分析. 兽类学报, 27 (3):298-307.
周智红. 2014. 近十年藏羚羊微卫星遗传多样性变化趋势. 西宁:青海师范大学硕士学位论文.
周智红, 郭松长, 马建滨, 徐永涛, 谭春敏, 都玉蓉. 2014. 藏羚羊 5
个微卫星标记的多态性分析. 青海师范大学学报 (自然科学版), 30 (2):36-39.
林婷婷. 2017. 大刺鳅 (Mastacembelus armatus) 微卫星标记开发及野生群体遗传多样性分析. 广州:广州大学硕士学位论文.
苗紫燕, 吴彤, 陈佳萍, 王东, 李宏奇, 连新明. 2020. 青藏铁路对雄性藏羚行为时间分配的影响. 兽类学报, 40 (2):135-142.
胡江. 2001. 野牦牛、家牦牛及其杂交种的生物学特性和遗传多样性研究. 兰州:甘肃农业大学硕士学位论文.
洪艳云, 李迪强, 易图永, 张于光, 刘毅. 2008. 筛选普氏原羚粪便DNA 中微卫星引物并应用于个体识别. 中国农学通报, 24 (9):54-58.
郭柯. 1993. 青海可可西里地区的植被. 植物生态学与地植物学学报, (2):26-38.
靳勇超. 2012. 基于粪便分子生物学对马鹿种群遗传结构以及家域的研究. 哈尔滨:东北林业大学硕士学位论文.
赛青高娃, 李宏奇, 王东, 王子涵, 连新明. 2022. 三江源国家公园迁徙藏羚对野生动物通道的利用:以青藏铁路五北大桥为例. 兽类学报, 42 (5):601-608.

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