为研究塔里木马鹿（Cervus elaphus yarkandensis）干旱环境适应中可能具有抗氧化功能的过氧化物还原酶1（Peroxiredoxins 1，PRDX1）的结构与功能特征，本研究通过逆转录PCR技术（RT‑PCR）扩增获取塔里木马鹿PRDX1（CeyPRDX1）基因的编码序列（CDS），使用同源重组法将其与pcDNA3.1(+) 载体相连接，并构建其真核表达载体，命名为pcDNA3.1(+)‑CeyPRDX1；利用具有EGFP荧光标签的pLJM1‑EGFP慢病毒骨架载体构建慢病毒过表达载体（pLJM1‑CeyPRDX1‑EGFP），并感染人永生化角质形成细胞株HaCaT后检测其亚细胞定位。对CeyPRDX1与其他哺乳动物的PRDX1基因序列进行对位排列，构建系统进化树；利用生物信息学方法对CeyPRDX1蛋白理化性质、跨膜结构、修饰位点和亚细胞定位等进行分析。结果显示，CeyPRDX1基因的CDS序列为729 bp，成功构建其真核表达载体和慢病毒过表达载体；亚细胞定位显示，CeyPRDX1定位于细胞质和细胞核中。对外排列和系统进化树分析结果显示，CeyPRDX1与东欧马鹿（Cervus elaphus）以及马鹿（Cervus canadensis）的同源性最高，亲缘关系最近。生物信息学分析结果显示，CeyPRDX1是高度保守的不稳定蛋白，没有N‑糖基化位点和跨膜区，其蛋白与多种抗氧化相关蛋白之间存在相互作用。本研究结果为后续CeyPRDX1基因的功能研究奠定了基础。

To investigate the structural and functional characteristics of Peroxiredoxin 1 (PRDX1)，which may play an antioxidant role in the adaptation of the Tarim red deer (Cervus elaphus yarkandensis) to arid environments, this study amplified the coding sequence (CDS) of the PRDX1 gene (CeyPRDX1) using reverse transcription PCR (RT-PCR). The CDS sequence was successfully cloned into the pcDNA3.1 (+) vector to construct an expression vector and named pcDNA3.1 (+) -CeyPRDX1. The pLJM1-EGFP Lentivirus vector with EGFP fluorescent label was used to construct overexpression vector of CeyPRDX1 (pLJM1-CeyPRDX1-EGFP), and its subcellular localization was observed in the cytoplasm and nucleus of human HaCaT cells. The results of homology comparison and phylogenetic tree analysis indicated that CeyPRDX1 has the highest homology and the closest phylogenetic relationship with Eastern European red deer (Cervus elaphus) and red deer (Cervus canadensis). Bioinformatic analysis revealed that CeyPRDX1 is a conserved, hydrophilic protein without N-glycosylation or transmembrane regions, and interacts with various antioxidant proteins. This study provides a foundation for future functional research on the CeyPRDX1 gene.