联合测定血浆纤维蛋白原与血清铁对瓶鼻海豚感染性疾病的诊断价值

黄泽林; 陈小芬; 张坤; 杨乃才; 沈博; 李建梁; 张鹏; 董贵信

doi:10.16829/j.slxb.150885

兽类学报 >

2026 , Vol. 46 >Issue 4: 610 - 616

DOI: https://doi.org/10.16829/j.slxb.150885

研究简报

联合测定血浆纤维蛋白原与血清铁对瓶鼻海豚感染性疾病的诊断价值

黄泽林 ,
陈小芬 ,
张坤 ,
杨乃才 ,
沈博 ,
李建梁 ,
张鹏 ,
董贵信

展开

1 长隆海洋王国, 珠海 519031;
2 广东华南珍稀野生动物物种保护中心, 珠海 519031

黄泽林（1972-），男，本科，兽医师，主要从事海洋动物临床诊疗工作.E-mail：linhzl@126.com

收稿日期: 2023-12-05

修回日期: 2024-07-25

网络出版日期: 2026-07-30

基金资助

广东省海洋经济发展专项（GDNRC[2022]40）；南方海洋科学与工程省实验室（珠海）创新团队专项（311021006）

收起

The diagnostic value of plasma fibrinogen and serum iron in infectious diseases of common bottlenose dolphins

HUANG Zelin ,
CHEN Xiaofen ,
ZHANG Kun ,
YANG Naicai ,
SHEN Bo ,
LI Jianliang ,
ZHANG Peng ,
DONG Guixin

Expand

1 Chimelong Ocean Kingdom, Zhuhai 519031, China;
2 Southern China Wildlife Species Conservation Center, Zhuhai 519031, China

Received date: 2023-12-05

Revised date: 2024-07-25

Online published: 2026-07-30

Fold

本文引用格式

黄泽林 , 陈小芬 , 张坤 , 杨乃才 , 沈博 , 李建梁 , 张鹏 , 董贵信 . 联合测定血浆纤维蛋白原与血清铁对瓶鼻海豚感染性疾病的诊断价值[J]. 兽类学报, 2026 , 46(4) : 610 -616 . DOI: 10.16829/j.slxb.150885

Abstract

This study focuses on the core technical problems of finding the inflammatory indicators in the bacterial infection of common bottlenose dolphin in Marine veterinary clinics. By analyzing 50 cases of treatment of bacterial infectious diseases in common bottlenose dolphin and 40 cases of healthy dolphins over a 10-year period, we have successfully established a new method for diagnosing bacterial infectious diseases in common bottlenose dolphin based on in-depth comparative analysis of plasma fibrinogen (FIB) and serum iron (SI). The value of the combined determination of FIB and SI in the diagnosis of bacterial diseases was confirmed. The results showed that the FIB reference of healthy common bottlenose dolphin was (2. 05 ±0. 39) g/L and the SI reference was (33. 45 ±12. 47) μmol/L. We also found that the FIB level of different infection groups was significantly different, and the severe bacterial infection group was higher than the general bacterial infection group (t=6. 337, P<0. 05). The SI level of the infected group was significantly lower than that of the healthy group (t=17. 569, P<0. 05；t=9. 811, P<0. 05), and the severe infection group was lower than that of the general infection group (t=9. 528, P<0. 05). Pearson correlation test showed that the correlation between FIB and SI of the three groups was very low. Our study shows that FIB and SI can assist in the diagnosis of bacterial infectious diseases in common bottlenose dolphins. FIB can be used as an early marker to better determine whether bacterial infection exists, while SI is helpful but cannot be diagnosed alone. Combined detection can improve the sensitivity and specificity of diagnosis and reduce the misdiagnosis rate. In bacterial infection, FIB is more sensitive and accurate, and dynamic combined detection has good diagnostic and clinical application value, providing strong technical support for cetacean protection.

Key words： Plasma fibrinogen; Serum iron; Common bottlenose dolphin; Bacterial infection; Diagnostic value

参考文献

American College of Chest Physicians,Society of Critical-Care Medicine Consensus Conference, 1992. Definitions for sepsis and multiple organ failure and guidelines for the use of innovative therapies in sepsis[J]. Critical Care Medicine, 20 (6):864-874. DOI:10.1097/00003246-199206000-00025.
Barratclough A,Gomez F M,Morey J S,Meegan J M,Parry C, Schwacke L,Jensen E D,Smith C R,2021. Biochemical and hematological biomarkers of reproductive failure in bottlenose dolphins Tursiops truncatus[J]. Diseases of Aquatic Organisms, 144:197-208. DOI:10.3354/dao03591.
Chang Jian, Zhang Yanrui, Qi Xin, 2016. Analysis of the mechanism coagulopathy in traumatic brain injury patients complicated with pulmonary infection[J]. Chinese Journal of the Frontiers of Medical Science, 8 (7):103-106. (in Chinese with English abstract)
Chifman J, Laubenbacher R, Torti S V, 2014. A systems biology approach to iron metabolism[J]. Advances in Experimental Medicine and Biology, 844:201-225. DOI:10.1007/ 978-1-4939-2095-2_10.
Da Silva C B,Wolkmer P,Paim F C,DaSilva A S,Siqueira L C, de Souza C L,França R T,Dornelles G L,Duarte M M M F, Monteiro S G, Mazzanti C M, dos Anjos Lopes S T, 2013. Iron metabolism and its relationship to anemia and immune system in Trypanosoma evansi infected rats[J]. Experimental Parasitology, 133 (3):357-364. DOI:10.1016/j. exppara. 2013. 01. 012.
Dierauf L A,Gulland F M D,2001. CRC handbook of marine mammal medicine:health,disease,and rehabilitation[M]. 2nd ed. Boca Raton, Florida:CRC Press, 240-423. DOI:10.1201/ 9781420041637.
Dierauf L A,Gulland F M D,2001. CRC handbook of marine mammal medicine:health,disease,and rehabilitation[M]. 2nd ed. Boca Raton, Florida:CRC Press, 309-328. DOI:10.1201/ 9781420041637.
Erskine R J, Bartlett P C, 1993. Serum concentrations of copper, iron,and zinc during Escherichia coli-induced mastitis[J]. Journal of Dairy Science, 76 (2):408-413. DOI:10.3168/jds. S0022-0302 (93) 77414-5.
Flower J E,Langan J N,Wells R S,Cray C,Arheart K L E,Chinnadurai S K, Adkesson M J, 2020. Hematologic and biochemical reference intervals for captive adult red pandas (Ailurus fulgens)[J]. Journal of Zoo and Wildlife Medicine, 51 (3):657-662. DOI:10.1638/2019-0175. 1.
Grieger T A,Kluger M J,1978. Fever and survival:the role of serum iron[J]. J Physiol (Lond), 279:187-196. DOI:10.1113/jphysiol. 1978. sp012247.
Henche M A L, Romero M C, Llorente G C, 1990. Levels of oligo-elements and trace elements in patients at the time of admission in intensive care units[J]. Nutrición Hospitalaria, 5 (4):338-345. DOI:10.3305/nh. 1990. 5. 4. 338.
Li Q T,Zhu J Y,Guo X K,2004. Hepeidin,iron metabolism and infection[J].Foreing Medical Sciences,27 (6):20-21. DOI:10.3969/j. issn. 1673-2588. 2004. 06. 006.
Li Xuening,Liu Ying, Cheng Xiaojun,Cheng Shikai,Fu Dajun, 2018. Clinical value of serum iron in the diagnosis and treatment of children with pulmonary infectious diseases[J]. Chinese Journal of Primary Medicine and Pharmacy, 25 (7):4. DOI:10.3760/cma. j. issn. 1008-6706. 2018. 07. 004. (in Chinese with English abstract)
Lu Y,Liu X Y,Chen Y J,Yu J,Yin S J,2015. Serum iron and A (2) DS (2) score in stroke-associated pneumonia[J]. Int J Clin Exp Med, 8 (4):6163-6170. DOI:10.1177/ 1940782415583438.
Payne S, Finkelstein R, 1978. The critical role of iron in host-bacterial interactions[J]. Journal of Clinical Investigation, 61 (6):1428-1440. DOI:10.1172/JCI108793.
Pi H,Jones S A,Mercer L E,Meador J P,Caughron J E,Jordan L,Newton S M,Conway T,Klebba P E,2012. Role of catecholate siderophores in gram-negative bacterial colonization of the mouse gut[J]. PLoS ONE, 7 (11):e50020. DOI:10.1371/journal. pone. 0050020.
Shi Yuexian,Zhao Yue,Hou Yahong,Gao Min,Wang Lijun,Shang Shaomei,2018. A study for non-disease factors correlated with hyperphosphatemia in patients receiving hemodialysis[J]. Chin J Nurs,53 (10):1186-1191. DOI:10.3761/j. issn. 0254-1769. 2018. 10. 006. (in Chinese with English abstract)
Srinivas U, Abdulla M, Åkesson B, Öckerman P A, Braconier J H, Jeppsson B, 1988. Trace element alterations in infectious diseases[J]. Scandinavian Journal of Clinical and Laboratory Investigation,48 (6):495-502. DOI:10.3109/0036551880 9084690.
Tidière M, Colchero F, Staerk J, Adkesson M J, Andersen D H, Bland L,Böye M,Brando S,Clegg I,Cubaynes S,Cutting A,De Man D,Derocher A E,Dorsey C,Elgar W,Gaglione E,Hansen K A,Jungheim A,Kok J,Laule G,López Goya A, Miller L, Pawlowsky T M, Mucha K, Owen M A, Petersen S D, Pilfold N, Richardson D, Richardson E S, Sabo D, Sato N, Shellabarger W W, Skovlund C, Tomisawa K, Trautwein S E,Van Bonn W,Van Elk C,Van Fersen L,Wahlberg M,Zhang P J,Zhang X F,Conde D A,2023. Survival improvements of marine mammals in zoological institutions mirror historical advances in human longevity[J]. Proceedings of the Royal Society B:Biological Sciences, 290 (2009):20231895. DOI:10.1098/rspb. 2023. 1895.
Venn-Watson S, Daniels R, Smith C, 2012. Thirty-year retrospective evaluation of pneumonia in a bottlenose dolphin (Tursiops truncatus) population[J]. Diseases of Aquatic Organisms, 99(3):237-242. DOI:10.3354/dao02498.
Wang X J, Wang Z, Zhang Z T, Qiu X S, Chen Y X, 2020. Plasma fibrinogen as a diagnostic marker of infection in patients with nonunions[J]. Infection and Drug Resistance, 13:4003-4008. DOI:10.2147/IDR. S265397.
Zhang D L,Rouault T A,2018. How does hepcidin hinder ferroportin activity?[J]. Blood, 131 (8):840-842. DOI:10.1182/blood-2017-11-815946.
刘莹,曹军皓,2010.纤维蛋白原临床研究进展[J].华南国防医学杂志, 24 (1):75-76. DOI:10.13730/j. 1009-2595. 2010. 01. 007.
李雪宁,刘颖,成晓君,程士凯,付大军,2018.血清铁检测在儿童肺部感染性疾病诊疗中的价值[J].中国基层医药,25 (7):4. DOI:10.3760/cma. j. issn. 1008-6706. 2018. 07. 004.
何静,卢卫国,2012.联合测定血清 PCT与 CRP对感染性疾病的诊断价值[J].检验医学与临床,9 (15):2. DOI:10.3969/j.issn. 1672-9455. 2012. 15. 042.
沈坤堂,张志德,1994.微量元素与感染[J].国际外科学杂志, 2:90-92.
荒川泰行,李春元,1991.肝疾病与微量元素[J].日本医学介绍, 12 (4):3.
胡健颖,冯泰,2002.实用统计学[J].北京:北京大学出版社, 236-237.
施月仙,赵岳,侯亚红,高敏,王丽君,尚少梅,2018.血液透析患者并发高磷血症的非疾病性因素研究[J].中华护理杂志, 53 (10):1186-1191. DOI:10.3761/j. issn. 0254-1769. 2018. 10. 006.
洪俊峰,2018.纤维蛋白原与肺部感染的相关性研究[J].临床医药文献电子杂志, 5 (86):73. DOI:10.16281/j. cnki.jocml. 2018. 86. 056.
常剑,张岩睿,齐欣,2016.颅脑创伤合并肺部感染患者相关性凝血障碍发病机制分析[J].中国医学前沿杂志,8(7):103-106.

Options

文章导航

模态框（Modal）标题

本文引用格式

Abstract

参考文献