Abstract: The pattern of excretion of urinary steroids and the variation of plasma steroids during oestrus in female Giant panda were described, and the biological luteinizing hormone (LH-BIO) change was firstly reported.The urinary estradiol (E₂) levels were increased throughout the oestrus, the peak value was 280.4nmol/mgCr. (76.4ng/mgCr.) which was occured two days before the period of the maximum behavioural cestrus, then it declined to the basal level of pro-oestrus.The urinary E₂ peak was coincident with the peak value of 92.0 IU/mgCr. of the urinary LH-BIO, meanwile, the female Giant panda intimated and mated with the male.The urinary progesterone (P) level was rapidly increased in one day after the urinary E₂ and LH-BIO peaks, and it remained in a high concentration for 6 days, the mean value of P of the luteal phase was statisticly different from that of the pro-oestrus value (p<0.01) . The plasma E₂, LH-BIO and P have shown the similar changes to those in urine,It was demonstrated that the female Giant panda had a normal ovulation during this oestrus.

Key words: Giant panda, Ailuropoda melanoleuca, Esttradiol, E₂, Progesterone, P, Luteinizing hormone, LH, Sex behaviour, Endocrine

摘要： 本文首次报道了用黄体生成素的生物测定法测定大熊猫发情期黄体生成素的生物活性与雌二醇、孕酮的关系。雌性大熊猫在发情高潮后两天,尿中黄体生成素的生物活性与雌二醇同时出现峰值,并与雄性变配。随后尿中孕酮浓度迅速增长,其浓度远远大于发情前期的水平。生殖内分泌的变化证实:雌性大熊猫在发情期中有成熟的卵子排出。

关键词: 大熊猫, 雌二醇, 孕酮, 黄体生成素, 性行为, 内分泌