Abstract: In order to explore the effect of levonorgestrel-quinestrol (EP-1) on the reproductive systems of male Apodemus draco and Apodemus agrarius, individuals of the two species of mice were divided into three groups: 30mg/kg single-dose group, 30mg/kg multiple-dose group and control group. These mice were dissected 15 days or 45 days after the initial treatment and changes of testis, epididymis, seminal vesicle, sperm density, testosterone and testicular tissue morphology were assessed. The results show that, 15 days after treatments, the weights of testis, epididymis and seminal vesicle were significantly lower than those of the control group; the sperm density and testosterone levels decreased significantly; and the structure of seminiferous tubule was damaged. At 45 days after treatment, physiological indicators of each treated group continued to decline. However, compared with the physiological indicators at the 15th day, the differences were not significant at the day 45. The differences between the single-dose group and multiple-dose group were not significant. The results of this study showed that EP-1 has significant anti-fertility effect on the reproductive systems of male Apodemus draco and Apodemus agrarius, but there were no significant differences between single-dose groups and multiple-dose groups.

Key words: Anti-fertility, EP-1, Male Apodemus draco, Male Apodemus agrarius

摘要： 为了检测左炔诺孕酮-炔雌醚（EP-1）对雄性中华姬鼠和黑线姬鼠的不育效果, 将32只雄性中华姬鼠和30只雄性黑线姬鼠分为 30mg/kg 单剂量组、30mg/kg多剂量组和对照组, 15d 和 45d 后剖检, 比较睾丸、附睾、储精囊、精子密度、睾酮含量及睾丸组织形态的变化。结果发现：给药后第 15 天，两种试鼠的睾丸、附睾、储精囊的重量较对照组明显降低; 精子密度、睾酮含量显著下降; 曲细精管结构破坏明显。给药后第45天，处理组各生理指标继续下降，但与第15天相比差别不显著；单剂量组和多剂量组在两个时间点的差别并不显著。结果表明，EP-1对雄性中华姬鼠和黑线姬鼠的繁殖器官有显著抑制效果，单次给药与多次给药的不育效果差别不大。

关键词: 雄性中华姬鼠, 雄性黑线姬鼠, EP-1, 不育控制