ACTA THERIOLOGICA SINICA ›› 2022, Vol. 42 ›› Issue (4): 410-419.DOI: 10.16829/j.slxb.150605
• ORIGINAL PAPERS • Previous Articles Next Articles
Kang AN1,2,3, Mingfang BAO1,2,3, Baohui YAO1,2,3, Yukun KANG1,2,3, Yuchen TAN1,2,3, Yanli WANG1,2,3, Junhu SU1,2,3()
Received:
2021-09-02
Accepted:
2022-02-23
Online:
2022-07-30
Published:
2022-07-22
Contact:
Junhu SU
安康1,2,3, 包明芳1,2,3, 姚宝辉1,2,3, 康宇坤1,2,3, 谭宇尘1,2,3, 王艳莉1,2,3, 苏军虎1,2,3()
通讯作者:
苏军虎
作者简介:
安康 (1997- ),男,硕士研究生,主要从事草地保护研究.
基金资助:
CLC Number:
Kang AN, Mingfang BAO, Baohui YAO, Yukun KANG, Yuchen TAN, Yanli WANG, Junhu SU. Expression of MTNR1a and MTNR1b genes on HPG axis of male plateau zokor during the breeding and the non‑breeding seasons[J]. ACTA THERIOLOGICA SINICA, 2022, 42(4): 410-419.
安康, 包明芳, 姚宝辉, 康宇坤, 谭宇尘, 王艳莉, 苏军虎. MTNR1a和MTNR1b基因在繁殖期与非繁殖期雄性高原鼢鼠HPG轴上的表达[J]. 兽类学报, 2022, 42(4): 410-419.
基因 Genes | 引物序列 Primer sequence | 退火温度 Annealing temperature (℃) | 片段大小 Size (bp) |
---|---|---|---|
MTNR1a | F: 5´–TCTTCAGGTCAGATGGACGGT–3´ R: 5´–CCCCCAGCAAATGGCAAAAA –3´ | 53.5 | 125 |
MTNR1b | F: 5´–ACCCCTGTGGCAGCACAGTG –3´ R: 5´–CTGAGGGACGTATTCCTTTGC –3´ | 53.5 | 156 |
β–actin | F: 5´–TTGTGCGTGACATCAAAGAG –3´ R: 5´–ATGCCAGAAGATTCCATACC –3´ | 53.5 | 200 |
Table 1 Primers sequence for MTNR1a and MTNR1b of male plateau zokor
基因 Genes | 引物序列 Primer sequence | 退火温度 Annealing temperature (℃) | 片段大小 Size (bp) |
---|---|---|---|
MTNR1a | F: 5´–TCTTCAGGTCAGATGGACGGT–3´ R: 5´–CCCCCAGCAAATGGCAAAAA –3´ | 53.5 | 125 |
MTNR1b | F: 5´–ACCCCTGTGGCAGCACAGTG –3´ R: 5´–CTGAGGGACGTATTCCTTTGC –3´ | 53.5 | 156 |
β–actin | F: 5´–TTGTGCGTGACATCAAAGAG –3´ R: 5´–ATGCCAGAAGATTCCATACC –3´ | 53.5 | 200 |
时期 Period | 个体数 Number | 体重 Body mass (g) | 睾丸重 Testis weight (g) | 附睾重 Epididymis weight (g) | 精囊腺重 Seminal vesicle weight (g) |
---|---|---|---|---|---|
繁殖期 Breeding | 10 | 315.52 ± 9.97 | 0.98 ± 0.04* | 0.42 ± 0.02** | 1.37 ± 0.04** |
非繁殖期 Non‑breeding | 10 | 313.36 ± 7.09 | 0.12 ± 0.01 | 0.04 ± 0.01 | 0.04 ± 0.01 |
Table 2 Weight changes of gonad organs during the breeding and non?breeding seasons of plateau zokor
时期 Period | 个体数 Number | 体重 Body mass (g) | 睾丸重 Testis weight (g) | 附睾重 Epididymis weight (g) | 精囊腺重 Seminal vesicle weight (g) |
---|---|---|---|---|---|
繁殖期 Breeding | 10 | 315.52 ± 9.97 | 0.98 ± 0.04* | 0.42 ± 0.02** | 1.37 ± 0.04** |
非繁殖期 Non‑breeding | 10 | 313.36 ± 7.09 | 0.12 ± 0.01 | 0.04 ± 0.01 | 0.04 ± 0.01 |
Fig. 1 MTNR1a and MTNR1b mRNA in the hypothalamus, pituitary, and testis of plateau zokor during the breeding and non?breeding seasons. A: relative expression level of MTNR1a mRNA; B: relative expression level of MTNR1b mRNA. *P < 0.05; **P < 0.01
Fig. 2 Immunohistochemical staining of MTNR1a and MTNR1b in testicular of plateau zokor during the breeding and non?breeding seasons. A, D: immunohistochemical staining of MTNR1a; B, E: immunohistochemical staining of MTNR1b; C, F: control group (using PBS instead of the first antibody); SC: Sertoli cell; LC: Leydig cell; Spg: spermatogonia; pSpc: primary spermatocyte; sSpc: secondary spermatocyte; rSpd: round spermatid; eSpd: elongate spermatid
Fig. 3 The average optical density of immunopositive signal in MTNR1a (A) and MTNR1b (B) of plateau zokor testis during the breeding and the non?breeding seasons. **P < 0.01
Aleandri V, Vincenzo S, Alberto M. 1996. The pineal gland and reproduction. Human Reproduction Update, 2 (3): 225-235. | |
An X Y, Wang Y J, Li Y C, Jia G X, Yang Q E. 2020. Morphological features and regulation of seasonal spermatogenesis in plateau zokor (Eospalax baileyi). Acta Theriologica Sinica, 40 (5): 435-445. (in Chinese) | |
Ansel L, Bolborea M, Bentsen A H, Klosen P, Mikkelsen J D, Simonneaux V. 2010. Differential regulation of kiss1 expression by melatonin and gonadal hormones in male and female Syrian hamsters. Journal of Biological Rhythms, 25 (2): 81-91. | |
Axelrod J, Wurtman R J. 1968. Photic and neural control of indoleamine metabolism in the rat pineal gland. Advances in Pharmacology, 6 (Pt A): 157-166. | |
Barrett P, Maclean A, Davidson G, Morgan P J. 1996. Regulation of the Mel1a melatonin receptor mRNA and protein levels in the ovine pars tuberalis: evidence for a cyclic adenosine 3’,5Mel’‒monophosphate‒independent 1a receptor coupling and an autoregulatory mechanism of expression. Molecular Endocrinology, 10 (7): 892-902. | |
Cecon E, Liu L, Jockers R. 2019. Melatonin receptor structures shed new light on melatonin research. Journal of Pineal Research, 67 (4): e12606. | |
Chen J N, Zhang Q W, Wang Q, Wang Y Y, Ma Y J, Zhang Y, Zhao X X. 2020. Expression of MTNR1a and MTNR1b in different tissues and localization in testis of yak (Bos grunniens). Acta Theriologica Sinica, 40 (1): 64-70. (in Chinese) | |
Chen Y L, Zhang X H. 2005. The seasonal and diurnal changes of blood melatonin in different breeds of sheep. Journal of Domestic Animal Ecology, 26 (1): 39-42. (in Chinese) | |
Clemens J W, Jarzynka M J, Witt‑Enderby P A.2001.Down‑regulation of mt1 melatonin receptors in rat ovary following estrogen exposure. Life Sciences, 69 (1): 27-35. | |
Dardente H, Klosen P, Pévet P, Masson‑Pévet M. 2003. MT1 melatonin receptor mRNA expressing cells in the pars tuberalis of the European hamster: effect of photoperiod. Journal of Neuroendocrinology, 15 (8): 778-786. | |
Dardente H, Lomet D, Robert V, Decourt C, Beltramo M, Pellicer‑Rubio M T.2016.Seasonal breeding in mammals: from basic science to applications and back. Theriogenology, 86 (1): 324-332. | |
Deng S L, Wang Z P, Jin C, Kang X L, Batool A, Zhang Y, Li X Y, Wang X X, Chen S R, Chang C S. 2018. Melatonin promotes sheep Leydig cell testosterone secretion in a co‑culture with Sertoli cells. Theriogenology, 106: 170-177. | |
Dubocovich M L, Yun K, Al‑ghoul W M, Benloucif S, Masana M I. 1998. Selective MT2 melatonin receptor antagonists block melatonin‑mediated phase advances of circadian rhythms. The FASEB Journal, 12 (12): 1211-1220. | |
Fischer C, Mueller T, Pfeffer M, Wicht H, von Gall C, Korf H W. 2017. Melatonin receptor 1 deficiency affects feeding dynamics and pro‑opiomelanocortin expression in the arcuate nucleus and pituitary of mice. Neuroendocrinology, 105 (1): 35-43. | |
Frungieri M B, Mayerhofer A, Zitta K, Pignataro O P, Calandra R S, Gonzalez‑Calvar S I. 2005. Direct effect of melatonin on Syrian hamster testis: melatonin subtype 1a receptors, inhibition of androgen production, and interaction with the local corticotropin‐releasing hormone system. Endocrinology, 146 (3): 1541-1552. | |
Gillette M U, Mitchell J W. 2002. Signaling in the suprachiasmatic nucleus: selectively responsive and integrative. Cell and Tissue Research, 309 (1): 99-107. | |
Guerrero H Y, Gauer F, Schuster C, Pévet P, Masson‑Pévet M.2000.Melatonin regulates the mRNA expression of the mt1 melatonin receptor in the rat pars tuberalis. Neuroendocrinology, 71 (3): 163-169. | |
Hanon E A, Lincoln G A, Fustin J M, Dardente H, Masson‑Pévet M, Morgan P J, Hazlerigg D G. 2008. Ancestral TSH mechanism signals summer in a photoperiodic mammal. Current Biology, 18 (15): 1147-1152. | |
Hao W L, Wang D W, Ren F, Hu X F, Tian L, Li N, Liu X H. 2016. Fecal hormones imply different reproduction strategies of male Brandt’s voles born in different seasons. Acta Theriologica Sinica, 36 (4): 413-421. (in Chinese) | |
Huo S D, Chen S E, Long R J, Yang J T, Lu J X, Zang R X, Zhang T J, Abudureyimu A, Liu J I, Zhang G H, Zhao Y Q, Ma Z R. 2017. Protein and mRNA expression of follicle-stimulating hormone receptor and luteinizing hormone receptor during the oestrus in the yak (Bos grunniens). Reproduction in Domestic Animals, 52 (3): 477-482. | |
Izzo G, Francesco A, Ferrara D, Campitiello M R, Serino I, Minucci S. 2010. Expression of melatonin (MT1, MT2) and melatonin‐related receptors in the adult rat testes and during development. Zygote, 18 (3): 257-264. | |
Jin X, von Gall C, Pieschl R L, Gribkoff V K, Stehle J H, Reppert S M, Weaver D R. 2003. Targeted disruption of the mouse Mel (1b) melatonin receptor. Molecular and Cellular Biology, 23 (3): 1054-1060. | |
Jockers R, Maurice P, Boutin J A, Delagrange P. 2008. Melatonin receptors, heterodimerization, signal transduction and binding sites: what’s new? British Journal of Pharmacology, 154 (6): 1182-1195. | |
Keinath D A, Griscom H R, Andersen M D. 2014. Habitat and distribution of the Wyoming pocket gopher (Thomomys clusius). Journal of Mammalogy, 95 (4): 803-813. | |
Klosen P, Sébert M E, Rasri K, Laran‑Chich M P, Simonneaux V. 2013. TSH restores a summer phenotype in photoinhibited mammals via the RF‑amides RFRP3 and kisspeptin. The FASEB Journal, 27 (7): 2677-2686. | |
Kozioł K, Broda D, Romerowicz‑Misielak M, Nowak S, Koziorowski M. 2020. Melatonin concentration in peripheral blood and melatonin receptors (MT1 and MT2) in the testis and epididymis of male roe deer during active spermatogenesis. Theriogenology, 149: 25-37. | |
Kuş İ, Akpolat N, Özen O A, Songur A, Kavaklį A, Sarsįlmaz M. 2002. Effects of melatonin on Leydig cells in pinealectomized rat: an immunohistochemical study. Acta Histochemica, 104 (1): 93-97. | |
Lincoln G A, Clarke I J. 1994. Photoperiodically‑induced cycles in the secretion of prolactin in hypothalamo‒pituitary disconnected rams: evidence for translation of the melatonin signal in the pituitary gland. Journal of Neuroendocrinology, 6 (3): 251-260. | |
Liu C, Weaver D R, Jin X, Shearman L P, Pieschl R L, Gribkoff V K, Reppert S M. 1997. Molecular dissection of two distinct actions of melatonin on the suprachiasmatic circadian clock. Neuron, 19 (1): 91-102. | |
Masson‑Pévet M, Gauer F. 1994. Seasonality and melatonin receptors in the pars tuberalis in some long day breeders. Neurosignals, 3 (2): 63-70. | |
Meng S S, Li J G, Lin G H. 2010. Research progress on subterranean rodents’ visual system regulation of circadian rhythms. Sichuan Journal of Zoology, 29 (5): 174-177. (in Chinese) | |
Moore R Y. 1995. Neural control of the pineal gland. Behavioural Brain Research, 73 (1): 125-130. | |
Mukherjee A, Haldar C. 2014. Photoperiodic regulation of melatonin membrane receptor (MT1R) expression and steroidogenesis in testis of adult golden hamster, Mesocricetus auratus . Journal of Photochemistry and Photobiology B‑Biology, 140: 374-380. | |
Nishiwaki‑Ohkawa T, Yoshimura T. 2016. Molecular basis for regulating seasonal reproduction in vertebrates. Journal of Endocrinology, 229 (3): 117-127. | |
Pandi‑Perumal S R, Trakht I, Srinivasan V, Spence D W, Maestroni G J M, Zisapel N, Cardinali D P. 2008. Physiological effects of melatonin: role of melatonin receptors and signal transduction pathways. Progress in Neurobiology, 85 (3): 335-353. | |
Reiter R J, Rosales‑Corral S A, Liu X Y, Acuna‑Castroviejo D, Escames G, Tan D X. 2015. Melatonin in the oral cavity: physiological and pathological implications. Journal of Periodontal Research, 50 (1): 9-17. | |
Reppert S M, Weaver D R. 2001. Molecular analysis of mammalian circadian rhythms. Annual Review of Physiology, 63 (1): 647-676. | |
Shinomiya A, Shimmura T, Nishiwaki‑Ohkawa T, Yoshimura T. 2014. Regulation of seasonal reproduction by hypothalamic activation of thyroid hormone. Frontiers in Endocrinology, 5: 12. | |
Simonneaux V, Ancel C, Poirel V J, Gauer F. 2013. Kisspeptins and RFRP‑3 act in concert to synchronize rodent reproduction with seasons. Frontiers in Neuroscience, 7 (7): 22. | |
Simonneaux V, Ribelayga C. 2003. Generation of the melatonin endocrine message in mammals: a review of the complex regulation of melatonin synthesis by norepinephrine, peptides, and other pineal transmitters. Pharmacological Reviews, 55 (2): 325-395. | |
Su J H, Peng R, Nan Z B, Ji W H, Cai Z S. 2018. Age determination and composition analyses of plateau zokor (Eospalax baileyi) in Gannan meadow. Chinese Journal of Zoology, 53 (1): 46-54. (in Chinese) | |
Tabecka‑Lonczynska A, Mytych J, Solek P, Kulpa M, Koziorowski M. 2017. New insight on the role of melatonin receptors in reproductive processes of seasonal breeders on the example of mature male European bison (Bison bonasus, Linnaeus 1758). Journal of Photochemistry and Photobiology B‑Biology, 173: 84-91. | |
Wang J J, Wang Y, Zhu M Y, Zhang F W, Sheng X, Zhang H L, Han Y Y, Yuan Z R, Weng Q. 2017. Seasonal expression of luteinizing hormone receptor and follicle stimulating hormone receptor in testes of the wild ground squirrels (Citellus dauricus Brandt). Acta Histochemica, 119: 727-732. | |
Wang Y J, Jia G X, Wang T, Wang X R, Liu Z H, Du Y R, Ma J B. 2020. Photoperiod‑melatonin signal regulates seasonal spermatogenesis in plateau pikas (Ochotona curzoniae). Acta Theriologica Sinica, 40 (1): 56-65. (in Chinese) | |
Wu Y H, Ursinus J, Zhou J N, Scheer F A J L, Ai‑Min B, Jockers R, Swaab D F. 2013. Alterations of melatonin receptors MT1 and MT2 in the hypothalamic suprachiasmatic nucleus during depression. Journal of Affective Disorders, 148 (2-3): 357-367. | |
Wurtman R J, Ozaki Y. 1978. Physiological control of melatonin synthesis and secretion: mechanisms, generating rhythms in melatonin, methoxytryptophol, and arginine vasotocin levels and effects on the pineal of endogenous catecholamines, the estrous cycle, and environmental lighting. Journal of Neural Transmission, 13 (13): 59-70. | |
Yang D P, He Y Q, Ge W B, Zhang Q W, Hua Dao C R, Wang X, Zhou K R, Chen W G. 2019. Expression analysis of MTR1a gene in hypothalamic‒pitutary‒ovarian axis during estrus cycle in ganjia tibetan sheep (Ovis aries). Journal of Agricultural Biotechnology, 27 (3): 457-463. (in Chinese) | |
Yang W C, Tang K Q, Fu C Z, Riaz H, Zhang Q, Zan L S. 2014. Melatonin regulates the development and function of bovine Sertoli cells via its receptors MT1 and MT2. Animal Reproduction Science, 147 (1-2): 10-16. | |
Yao W, Wang D H, Zhang X Y. 2017. Molecular basis of annual clock and photosensitive neural circuits for regulating seasonal reproduction in mammals. Chinese Journal of Zoology, 52 (4): 717-725. (in Chinese) | |
Zhang Y M, Liu J K. 2002. The biological characteristics of subterranean rodents and their roles in ecosystem. Acta Theriologica Sinica, 22 (2): 144-153. (in Chinese) | |
Zheng S W. 1980. Studies on reproduction in mole rat (Myospalax fontanierii Milne‑Edwards). Zoological Research, 1 (4): 465-477. (in Chinese) | |
安晓宇, 王玉军, 李永昌, 贾功雪, 杨其恩. 2020. 高原鼢鼠精子发生的形态学特征和关键调控因子探究. 兽类学报, 40 (5): 435-445. | |
陈健楠, 张全伟, 王琪, 王媛媛, 马友记, 张勇, 赵兴绪. 2020. MTNR1a和MTNR1b在牦牛不同组织中的表达及其在睾丸中的定位. 兽类学报, 40 (1): 64-70. | |
陈玉林, 张小辉. 2005. 不同绵羊品种褪黑激素的季节性及昼夜变化规律研究. 家畜生态学报, 26 (1): 39-42. | |
郝伟丽, 王大伟, 任飞, 胡祥发, 田林, 李宁, 刘晓辉. 2016. 粪便激素水平反映不同出生时期雄性布氏田鼠的繁殖策略. 兽类学报, 36 (4): 413-421. | |
孟衫衫, 李金钢, 林恭华. 2010. 地下鼠视觉系统对昼夜节律调节研究进展. 四川动物, 29 (5): 174-177. | |
苏军虎, 彭然, 南志标, JI Weihong, 蔡卓山. 2018. 甘南草原高原鼢鼠年龄划分及其组成分析. 动物学杂志, 53 (1): 46-54. | |
王玉军, 贾功雪, 王婷, 王秀蓉, 刘忠浩, 都玉蓉, 马建滨. 2020. 光周期—褪黑素信号调控高原鼠兔精原细胞分化和季节性精子发生. 兽类学报, 40 (1): 56-65. | |
杨大鹏, 何玉琴, 葛闻博, 张全伟, 华道才让, 王霞, 周凯仁, 陈卫刚. 2019. 甘加藏羊发情周期下丘脑—垂体—卵巢轴中MTR1a基因的表达分析. 农业生物技术学报, 27 (3): 457-463. | |
姚蔚, 王德华, 张学英. 2017. 哺乳动物季节性繁殖的内源年生物钟及光敏神经环路研究进展. 动物学杂志, 52 (4): 717-725. | |
张堰铭, 刘季科. 2002. 地下鼠生物学特征及其在生态系统中的作用. 兽类学报, 22 (2): 144-153. | |
郑生武. 1980. 中华鼢鼠的繁殖研究. 动物学研究, 1 (4): 465-477. |
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