In order to explore the effect of levonorgestrel-quinestrol (EP-1) on the reproductive systems of male Apodemus draco and Apodemus agrarius, individuals of the two species of mice were divided into three groups: 30mg/kg single-dose group, 30mg/kg multiple-dose group and control group. These mice were dissected 15 days or 45 days after the initial treatment and changes of testis, epididymis, seminal vesicle, sperm density, testosterone and testicular tissue morphology were assessed. The results show that, 15 days after treatments, the weights of testis, epididymis and seminal vesicle were significantly lower than those of the control group; the sperm density and testosterone levels decreased significantly; and the structure of seminiferous tubule was damaged. At 45 days after treatment, physiological indicators of each treated group continued to decline. However, compared with the physiological indicators at the 15th day, the differences were not significant at the day 45. The differences between the single-dose group and multiple-dose group were not significant. The results of this study showed that EP-1 has significant anti-fertility effect on the reproductive systems of male Apodemus draco and Apodemus agrarius, but there were no significant differences between single-dose groups and multiple-dose groups.