The study of gas environment in plateau zokor’s tunnels is very important for understanding the hypoxia tolerance mechanism of the zokor. We selected active tunnels with zokor, inactive tunnels without zokor and the soil in the same layer in areas surrounding zokor’s tunnels on alpine meadow in the eastern Qilian Mountain region. Using Soil Gas Monitors in situ for 12 months, we measured gas content of oxygen, carbon dioxide, methane, and temperature inside the tunnels and soil, as well as the gas content and temperature in the air aboveground. Based on One-Way ANOVA , General linear model-Repeated Measures and Pearson analysis, our results showed that the oxygen content inside the zokor’s tunnel was less than that of inactive tunnel, soil and air aboveground during every month except October and November（P<0.05）,the carbon dioxide content inside active tunnels was greater than that of inactive tunnels, soil and air aboveground during every month except July and August, the methane content inside active tunnels was greater than that of air aboveground every month; however, the monthly difference varied compared to inactive tunnels and soil. The low peak value of oxygen and high peak value of carbon dioxide inside the zokor’s tunnel occurred in May and September, these values for inactive tunnels, soil and air aboveground air appeared from June to August, the low and high peak value of methane inside active tunnels, inactive tunnels, soil and air aboveground appeared in January and December, and May and September, respectively. The results showed that significant differences existed for months, treatments and the interaction between months and treatments(P<0.05). So Plateau zokor lived in habitat with low oxygen, high carbon dioxide and higher methane content, gas of tunnel also were affected by season and Plateau zokor’s activities.