Endothelin-1 is a potent vasoconstricting factor that can induce structural and functional changes of the blood vessel by promoting vascular smooth muscle cell proliferation. This study was designed to study the molecular basis of high-altitude adaption in yak with a focus on the potential role of Endothelin-1. We cloned and analyzed the CDS of yak (Bos grunniens) Endothelin-1 and constructed a eukaryotic expression plasmid to provide a useful tool to study its fuction in the future. The results showed that the full-length of yak Endothelin-1 contains a complete ORF (609bp) encoding 202 amino acids. The putative molecular weight and theory isoelectric point of Endothelin-1 gene in yak are 22.98 kDa and 9.63. We also successfully constructed a eukaryotic expression vector pEGFP-C1-Endothelin-1 in which the CDS of the Endothelin-1 is driven by CMV promoter and the expression can be monitored by GFP. Amino acid sequences of yak Endothelin-1 are 100%、93%、81%、77%、70% identical to those in Bos Taurus、Ovis aries、Pantholops hodgsoni and Homo sapiens, respectively. In summary, findings from the study will pave the way to further explore the molecular mechanism of high-altitude adaption in yak and likely other animals.