The spatial utilization and activity pattern characteristics of wild animals during reproduction are specific manifestations of long-term adaptation to the environment in which they live, and are among the most important basic issues in animal ecology research. However, few studies have focused on the behavioral strategies adopted by individual animals in spatial use at different spatial and temporal scales. From March 2010 to March 2012, using GPS collars equipped with dual-axis activity sensors, we recorded the activity locations and activity level of a female wild giant panda (Ailuropoda melanoleuca) which conceived and gave birth to a cub. We studied the spatial use and activity patterns of the giant panda in different reproductive stages (period after mating and conception, cub-bearing period and breeding period). Results show that, in the breeding period (March 2010 to March 2011), the main utilization space of female giant panda was 2.13km2; during the period after mating and conception (from April to June), the range of activity, average daily movement distance and activity level of the female giant panda increased considerably; around the cub-bearing period (from August to September), the range of activity of the female giant panda was distinctly reduced, and the average daily movement distance and activity intensity were visibly reduced. In winter, the range of activity, average daily movement distance and activity level of the female giant panda gradually increased. In the breeding period (March 2011 to March 2012), the main utilization space of the female giant panda with the cub was 2.31km2, which was slightly higher than that in the period of cub-bearing. The average daily movement distance and activity intensity of the giant panda were lower than that in the spring of the period of cub-bearing, however, the average daily movement distance and activity intensity of giant panda were considerably higher in summer, autumn and winter than that in the same season of the previous year. Results of our study can provide a scientific basis for the formulation of policies on the protection and management of giant pandas and their habitats during the breeding period.