As a newly developed mega-city, the biodiversity of Shenzhen suffer the influence from urbanization in a shorter period than which of traditional mega-cities. However, there is a few comprehensive survey for the biological resources in this city. In order to discover the current situation of animals, we set up 57 camera traps in Shenzhen from April 27th, 2014 to April 5th, 2015. Based on the survey effort of 3780 camera-trap days and 607 independent valid photos, we found 8 species of mammals belonging to 3 orders and 6 families, and 26 species of birds belonging to 3 orders, 9 families, including a second-class state protected species Centropus sinensis, and 24 species listed in the National Protection of Beneficial or Important Economic and Scientific Research Value of Terrestrial Wildlife. Except for mice, there are also some species with high Relative Abundance Index like Turdus hortulorum, Sus scrofa, Zoothera citrina, Turdus cardis. And we found that the daily activity pattern of wild animals in metropolitan area is different to which in natural reserves. This study would provide a new ideal and a new method for further research and protection of animal resources of Shenzhen.